TY - JOUR
T1 - Selective inhibition of chlorophyll biosynthesis by nicotinamide
AU - Shioi, Yuzo
AU - Doi, Michio
AU - Böddi, Béla
N1 - Funding Information:
i This study was supported in part by a Grant-in Aid for Scientific Research from the Japanese Ministry of Education, Science and Culture. ’ To whom correspondence should be addressed. a Permanent address: Department of Plant Physiology, E&v& Lorind University, Budapest, Hungary. 4 Abbreviations used: Bchl, bacteriochlorophyll; DV, divinyl; MV, monovinyl; Pchlide, protochlorophyllide (mixture of monovinyl and divinyl proto-chlorophyllides).
Funding Information:
The authors thank the Yamada Science Foundation for supplying the funds to B.B. for study Japan.
PY - 1988/11/15
Y1 - 1988/11/15
N2 - Rhodobacter sphaeroides grown in the presence of nicotinamide excreted bacteriochlorophyll precursors, 2,4-divinyl protochlorophyllide (DV-Pchlide) and a small amount of 2-monovinyl protochlorophyllide (MV-Pchlide). Accumulation of these pigments indicates that nicotinamide inhibited the bacteriochlorophyll biosynthetic pathway site-specifically between DV-Pchlide and MV-Pchlide. This phenomenon is also observed in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. Among 12 nicotinamide derivatives and isomers tested, only nicotinamide was effective, indicating that in addition to the completeness of the pyridine ring skeleton at positions 1 to 3, the carboxylic acid amide group is essential for this inhibition. The technique described in this report permits the simple preparation of large quantities of DV-Pchlide.
AB - Rhodobacter sphaeroides grown in the presence of nicotinamide excreted bacteriochlorophyll precursors, 2,4-divinyl protochlorophyllide (DV-Pchlide) and a small amount of 2-monovinyl protochlorophyllide (MV-Pchlide). Accumulation of these pigments indicates that nicotinamide inhibited the bacteriochlorophyll biosynthetic pathway site-specifically between DV-Pchlide and MV-Pchlide. This phenomenon is also observed in an aerobic photosynthetic bacterium, Erythrobacter sp. OCh 114. Among 12 nicotinamide derivatives and isomers tested, only nicotinamide was effective, indicating that in addition to the completeness of the pyridine ring skeleton at positions 1 to 3, the carboxylic acid amide group is essential for this inhibition. The technique described in this report permits the simple preparation of large quantities of DV-Pchlide.
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U2 - 10.1016/0003-9861(88)90009-4
DO - 10.1016/0003-9861(88)90009-4
M3 - Article
C2 - 2973768
AN - SCOPUS:0024289340
SN - 0003-9861
VL - 267
SP - 69
EP - 74
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
IS - 1
ER -