Selective nitration of psbo1 inhibits oxygen evolution from isolated arabidopsis thylakoid membranes

Misa Takahashi, Jun Shigeto, Atsushi Sakamoto, Hiromichi Morikawa

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Treatment of isolated Arabidopsis thaliana thylakoid membranes with nitrogen dioxide (NO2) induces selective nitration of the tyrosine residue at the ninth amino acid (9Tyr) of PsbO1. This selective nitration is triggered by light and is inhibited by photosynthetic electron transport inhibitors. Therefore, we postulated that, similar to 161Tyr of D1 (YZ), 9Tyr of PsbO1 is redox active and is selectively oxidized by photosynthetic electron transport in response to illumination to a tyrosyl radical that is highly susceptible to nitration. This tyrosyl radical may combine rapidly at diffusion-controlled rates with NO2 to form 3- nitrotyrosine. If this postulation is correct, the nitration of 9Tyr of PsbO1 should decrease oxygen evolution activity. We investigated the effects of PsbO1 nitration on oxygen evolution from isolated thylakoid membranes, and found that nitration decreased oxygen evolution to ≥0% of the control. Oxygen evolution and nitration were significantly negatively correlated. This finding is consistent with redox active properties of the 9Tyr gene of PsbO1, and suggests that PsbO1 9Tyr acts as an electron relay, such as YZ in the photosystem II oxygenic electron transport chain.

Original languageEnglish
Article numbere1304342
JournalPlant Signaling and Behavior
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 3 2017

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thylakoids
nitrogen dioxide
Arabidopsis
electron transfer
electron transport chain
photosystem II
tyrosine
lighting
Arabidopsis thaliana
electrons
amino acids
oxygen production
genes

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

Selective nitration of psbo1 inhibits oxygen evolution from isolated arabidopsis thylakoid membranes. / Takahashi, Misa; Shigeto, Jun; Sakamoto, Atsushi; Morikawa, Hiromichi.

In: Plant Signaling and Behavior, Vol. 12, No. 4, e1304342, 03.04.2017.

Research output: Contribution to journalArticle

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