Selective protein separation using siliceous materials with a trimethoxysilane-containing glycopolymer

Hirokazu Seto, Yutaro Ogata, Tatsuya Murakami, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A copolymer with α-d-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)-immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)- immobilized membrane was recoverable by permeation of a mannose derivative at high concentration.

Original languageEnglish
Pages (from-to)411-417
Number of pages7
JournalACS Applied Materials and Interfaces
Volume4
Issue number1
DOIs
Publication statusPublished - Jan 25 2012

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

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