Abstract
A copolymer with α-d-mannose (Man) and trimethoxysilane (TMS) units was synthesized for immobilization on siliceous matrices such as a sensor cell and membrane. Immobilization of the trimethoxysilane-containing copolymer on the matrices was readily performed by incubation at high heat. The recognition of lectin by poly(Man-r-TMS) was evaluated by measurement with a quartz crystal microbalance (QCM) and adsorption on an affinity membrane, QCM results showed that the mannose-binding protein, concanavalin A, was specifically bound on a poly(Man-r-TMS)-immobilized cell with a higher binding constant than bovine serum albumin. The amount of concanavalin A adsorbed during permeation through a poly(Man-r-TMS)-immobilized membrane was higher than that through an unmodified membrane. Moreover, the concanavalin A adsorbed onto the poly(Man-r-TMS)- immobilized membrane was recoverable by permeation of a mannose derivative at high concentration.
Original language | English |
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Pages (from-to) | 411-417 |
Number of pages | 7 |
Journal | ACS Applied Materials and Interfaces |
Volume | 4 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 25 2012 |
All Science Journal Classification (ASJC) codes
- Materials Science(all)