Abstract
The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-Associated groups of RNA were readily resolved: Those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-Transported in developing rice seeds.
| Original language | English |
|---|---|
| Pages (from-to) | 5045-5058 |
| Number of pages | 14 |
| Journal | Journal of Experimental Botany |
| Volume | 69 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - Oct 12 2018 |
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All Science Journal Classification (ASJC) codes
- Physiology
- Plant Science
Cite this
Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant. / Yang, Yongil; Chou, Hong Li; Crofts, Andrew J.; Zhang, Laining; Tian, Li; Washida, Haruhiko; Fukuda, Masako; Kumamaru, Toshihiro; Oviedo, Oliver J.; Starkenburg, Shawn R.; Okita, Thomas W.
In: Journal of Experimental Botany, Vol. 69, No. 21, 12.10.2018, p. 5045-5058.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant
AU - Yang, Yongil
AU - Chou, Hong Li
AU - Crofts, Andrew J.
AU - Zhang, Laining
AU - Tian, Li
AU - Washida, Haruhiko
AU - Fukuda, Masako
AU - Kumamaru, Toshihiro
AU - Oviedo, Oliver J.
AU - Starkenburg, Shawn R.
AU - Okita, Thomas W.
PY - 2018/10/12
Y1 - 2018/10/12
N2 - The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-Associated groups of RNA were readily resolved: Those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-Transported in developing rice seeds.
AB - The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-Associated groups of RNA were readily resolved: Those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-Transported in developing rice seeds.
UR - http://www.scopus.com/inward/record.url?scp=85054896152&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85054896152&partnerID=8YFLogxK
U2 - 10.1093/jxb/ery297
DO - 10.1093/jxb/ery297
M3 - Article
C2 - 30102323
AN - SCOPUS:85054896152
VL - 69
SP - 5045
EP - 5058
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
SN - 0022-0957
IS - 21
ER -