@article{dd14a0daea8c43faa6d0028f722fe0b8,
title = "Selective sets of mRNAs localize to extracellular paramural bodies in a rice glup6 mutant",
abstract = "The transport of rice glutelin storage proteins to the storage vacuoles requires the Rab5 GTPase and its related guanine nucleotide exchange factor (Rab5-GEF). Loss of function of these membrane vesicular trafficking factors results in the initial secretion of storage proteins and later their partial engulfment by the plasma membrane to form an extracellular paramural body (PMB), an aborted endosome complex. Here, we show that in the rice Rab5-GEF mutant glup6, glutelin RNAs are specifically mislocalized from their normal location on the cisternal endoplasmic reticulum (ER) to the protein body-ER, and are also apparently translocated to the PMBs. We substantiated the association of mRNAs with this aborted endosome complex by RNA-seq of PMBs purified by flow cytometry. Two PMB-Associated groups of RNA were readily resolved: Those that were specifically enriched in this aborted complex and those that were highly expressed in the cytoplasm. Examination of the PMB-enriched RNAs indicated that they were not a random sampling of the glup6 transcriptome but, instead, encompassed only a few functional mRNA classes. Although specific autophagy is also an alternative mechanism, our results support the view that RNA localization may co-opt membrane vesicular trafficking, and that many RNAs that share function or intracellular location are co-Transported in developing rice seeds.",
author = "Yongil Yang and Chou, {Hong Li} and Crofts, {Andrew J.} and Laining Zhang and Li Tian and Haruhiko Washida and Masako Fukuda and Toshihiro Kumamaru and Oviedo, {Oliver J.} and Starkenburg, {Shawn R.} and Okita, {Thomas W.}",
note = "Funding Information: This work was supported by grants from the Japan Society for the Promotion of Science (TK and AJC), from the National Science Foundation MCB-1444610 and IOS-1701061, and from the USDA-NIFA Projects 0590 and 0119,Agricultural Research Center, College of Agricultural, Human, and Natural Resource Sciences, Washington State University.This article was written while the corresponding author (TWO) was serving as a program director at the National Science Foundation. Any opinions, findings and conclusions or recommendations expressed in this paper are those of the authors and do not necessarily reflect the views of the National Science Foundation. Author contributions: HLC and YY designed and conducted most of the experiments and analysed the data; AJC conducted the initial studies on the isolation of PMBs using seed peels and assisted in analysing the RNA-seq data; OJO and RS generated the transcriptome data for the various rice lines; LT and LZ also assisted in analysing the RNA-seq data; HW characterized the RNA localization properties of the glup6 mutant line; MF conducted the electron microscopy study;TK conducted the rice mutant population screens and characterization of the glup6 line;TWO supervised the research; HLC,YY, AJC, and TWO wrote the paper with critical inputs from the other co-authors. Publisher Copyright: {\textcopyright} {\textcopyright} The Author(s) 2018",
year = "2018",
month = oct,
day = "12",
doi = "10.1093/jxb/ery297",
language = "English",
volume = "69",
pages = "5045--5058",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "Oxford University Press",
number = "21",
}