@inbook{3c205f316b65458aa7d5c43347815dee,
title = "Selective Suppression of Endogenous Gene Expression Using RNAi in Drosophila Schneider S2 Cells",
abstract = "RNA interference (RNAi) is a posttranscriptional gene silencing method that is triggered by double-stranded RNA (dsRNA). RNAi is used to inactivate genes of interest and provides a genetic tool for loss-of-function studies in a variety of organisms. I have used this method to reveal the physiological roles of a number of endogenous proteins involved in mitochondrial DNA metabolism in Schneider cells, including the mitochondrial single-stranded DNA-binding protein. Here, I present experimental schemes of selective suppression of endogenous gene expression using RNAi in Drosophila Schneider S2 cells. With this method, the function of exogenous wild-type or mutant genes can be evaluated.",
author = "Yuichi Matsushima",
note = "Funding Information: I would like to thank Dr. Rebecca Porter from Edanz Group (www. edanzediting.com/ac) for editing a draft of this manuscript. This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Numbers 15H03096, 18H03180, and 18K19303. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1290-3_19",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "303--312",
booktitle = "Methods in Molecular Biology",
}