Selective Suppression of Endogenous Gene Expression Using RNAi in Drosophila Schneider S2 Cells

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA interference (RNAi) is a posttranscriptional gene silencing method that is triggered by double-stranded RNA (dsRNA). RNAi is used to inactivate genes of interest and provides a genetic tool for loss-of-function studies in a variety of organisms. I have used this method to reveal the physiological roles of a number of endogenous proteins involved in mitochondrial DNA metabolism in Schneider cells, including the mitochondrial single-stranded DNA-binding protein. Here, I present experimental schemes of selective suppression of endogenous gene expression using RNAi in Drosophila Schneider S2 cells. With this method, the function of exogenous wild-type or mutant genes can be evaluated.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages303-312
Number of pages10
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2281
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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