Thiolated heme derivatives were synthesized and their self-assembly behaviors on gold surfaces together with that of native heme were studied. The kinetics of this adsorption and the thickness of the formed monolayer were investigated by surface plasmon resonance spectroscopy. Native heme shows a different adsorption behavior compared to that of the synthesized derivatives. Though the heme derivatives were found to form monolayers, native heme formed only irregular aggregates on gold surfaces. IR spectroscopy was used to reveal the molecular structure of the adsorbates in the vicinity of the peptide linkage in the heme molecules on the gold surfaces. UV spectroscopy was employed to determine the tilt angle of the porphyrin rings relative to the gold surface normal. Cyclic voltammograms and potential-modulated UV-vis reflectance spectra of heme derivatives were observed in order to estimate the adsorbed amount and the redox behaviors of heme derivatives on gold surfaces.
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