Self-assembled nanowire arrays as three-dimensional nanopores for filtration of DNA molecules

Sakon Rahong, Takao Yasui, Takeshi Yanagida, Kazuki Nagashima, Masaki Kanai, Gang Meng, Yong He, Fuwei Zhuge, Noritada Kaji, Tomoji Kawai, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Molecular filtration and purification play important roles for biomolecule analysis. However, it is still necessary to improve efficiency and reduce the filtration time. Here, we show self-assembled nanowire arrays as three-dimensional (3D) nanopores embedded in a microfluidic channel for ultrafast DNA filtration. The 3D nanopore structure was formed by a vapor-liquid-solid (VLS) nanowire growth technique, which allowed us to control pore size of the filtration material by varying the number of growth cycles. λ DNA molecules (48.5 kbp) were filtrated from a mixture of T4 DNA (166 kbp) at the entrance of the 3D nanopore structure within 1 s under an applied electric field. Moreover, we observed single DNA molecule migration of T4 and λ DNA molecules to clarify the filtration mechanism. The 3D nanopore structure has simplicity of fabrication, flexibility of pore size control and reusability for biomolecule filtration. Consequently it is an excellent material for biomolecular filtration.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
Journalanalytical sciences
Volume31
Issue number3
DOIs
Publication statusPublished - 2015

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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