Self-assembly of α-helix peptide/crown ether conjugate upon complexation with ammonium-terminated alkanethiolate

Yoshiko Miura, Shunsaku Kimura, Yukio Imanishi, Junzo Umemura

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Hydrophobic helical crowned peptides were synthesized and investigated on formation of an oriented thin layer of the helix peptides. The helix peptides in ethanol were incubated with a self-assembled monolayer (SAM) of ammonium-terminated alkanethiolate on a gold surface. The thickness of the peptide thin layer formed on the gold surface was determined by the surface plasmon resonance method. Complexation of the crowned peptide with the ammonium-terminated alkanethiolate facilitated formation of a peptide monolayer or multilayers on gold depending on the peptide concentration. The orientation of the helix peptide on the gold surface was investigated by FT-IR reflection-absorption spectroscopy. The tilt angle of the helix axis from the normal of the gold surface was estimated to be 28°, when the crowned peptide complexed with the SAM of N-(∈-aminocaproyl)aminoethyl disulfide. This degree of orientation is more vertical to the surface than that of the complexed peptide with a SAM of 2-aminoethanethiol hydrochloride. Since the helix peptide without the crown ether unit was oriented in parallel to the surface, the crown ether/ammonium complexation should promote the vertical orientation of the helix peptides on the gold surface.

Original languageEnglish
Pages (from-to)2761-2767
Number of pages7
JournalLangmuir
Volume14
Issue number10
Publication statusPublished - May 12 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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