Self-assembly of mastoparan X derivative having fluorescence probe in lipid bilayer membrane

Katsuhiko Fujita, Shunsaku Kimura, Yukio Imanishi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A mastoparan X (MPX) derivative having an anthryl group at the C-terminal residue was synthesized (MPX-A), and its conformation, orientation and aggregation in phospholipid bilayer membrane were studied. The efficiency of intramolecular energy transfer from the Trp residue to the anthryl group at high peptide dilution suggested α-helical conformation in the lipid membrane, which is consistent with the previous report by NMR of MPX concentrated in the membrane. Either emission from the Trp residue or the anthryl group of MPX-A in the lipid membrane was quenched by 5-doxylstearic acid, suggesting that MPX-A is located at the membrane surface with the helix axis oriented parallel to the surface. The dependence of the excited energy transfer and the fluorescence depolarization of MPX-A on the peptide concentration revealed that MPX-A aggregated in the lipid membrane to form a defined structure.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalBBA - Biomembranes
Volume1195
Issue number1
DOIs
Publication statusPublished - Oct 12 1994
Externally publishedYes

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Lipid bilayers
Lipid Bilayers
Membrane Lipids
Self assembly
Fluorescence
Derivatives
Membranes
Energy Transfer
Energy transfer
Conformations
Peptides
Depolarization
mastoparan X
Crystal orientation
Dilution
Phospholipids
Agglomeration
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Self-assembly of mastoparan X derivative having fluorescence probe in lipid bilayer membrane. / Fujita, Katsuhiko; Kimura, Shunsaku; Imanishi, Yukio.

In: BBA - Biomembranes, Vol. 1195, No. 1, 12.10.1994, p. 157-163.

Research output: Contribution to journalArticle

Fujita, Katsuhiko ; Kimura, Shunsaku ; Imanishi, Yukio. / Self-assembly of mastoparan X derivative having fluorescence probe in lipid bilayer membrane. In: BBA - Biomembranes. 1994 ; Vol. 1195, No. 1. pp. 157-163.
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