Abstract
We previously described the mass preparation of hepatocyte spheroids in a cell suspension. Addition of a synthetic polymer, Eudragit S100 (a co-polymer of methacrylic acid and methylmethacrylate), to the culture medium as an artificial matrix has been found to promote cell aggregation and induce spheroid formation. The present study examines co-cultures of hepatocytes and liver non-parenchymal cells to further enhance the stable expression of liver functions. Hetero-spheroids consisting of hepatocytes and non-parenchymal cells were also formed by adding the polymer to the culture medium. The expression levels of liver functions depended on the mixing ratio of the cells. The values of albumin secretion, ammonia removal and urea synthesis were the highest when equal number of hepatocytes and non-parenchymal cells were mixed. The hetero-spheroids maintained a higher level of albumin secretion for over 4 weeks compared with co-cultured cells without the polymer and spheroids consisting of hepatocytes solely. Other liver functions such as ammonia removal and cytochrome P-450 activity in hetero-spheroid cultures were also maintained at a high level throughout the 4-week culture period.
| Original language | English |
|---|---|
| Pages (from-to) | 135-143 |
| Number of pages | 9 |
| Journal | Biochemical Engineering Journal |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 14 2001 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Environmental Engineering
- Biomedical Engineering
Cite this
Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture. / Yamada, Keisuke; Kamihira, Masamichi; Iijima, Shinji.
In: Biochemical Engineering Journal, Vol. 8, No. 2, 14.08.2001, p. 135-143.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Self-organization of liver constitutive cells mediated by artificial matrix and improvement of liver functions in long-term culture
AU - Yamada, Keisuke
AU - Kamihira, Masamichi
AU - Iijima, Shinji
PY - 2001/8/14
Y1 - 2001/8/14
N2 - We previously described the mass preparation of hepatocyte spheroids in a cell suspension. Addition of a synthetic polymer, Eudragit S100 (a co-polymer of methacrylic acid and methylmethacrylate), to the culture medium as an artificial matrix has been found to promote cell aggregation and induce spheroid formation. The present study examines co-cultures of hepatocytes and liver non-parenchymal cells to further enhance the stable expression of liver functions. Hetero-spheroids consisting of hepatocytes and non-parenchymal cells were also formed by adding the polymer to the culture medium. The expression levels of liver functions depended on the mixing ratio of the cells. The values of albumin secretion, ammonia removal and urea synthesis were the highest when equal number of hepatocytes and non-parenchymal cells were mixed. The hetero-spheroids maintained a higher level of albumin secretion for over 4 weeks compared with co-cultured cells without the polymer and spheroids consisting of hepatocytes solely. Other liver functions such as ammonia removal and cytochrome P-450 activity in hetero-spheroid cultures were also maintained at a high level throughout the 4-week culture period.
AB - We previously described the mass preparation of hepatocyte spheroids in a cell suspension. Addition of a synthetic polymer, Eudragit S100 (a co-polymer of methacrylic acid and methylmethacrylate), to the culture medium as an artificial matrix has been found to promote cell aggregation and induce spheroid formation. The present study examines co-cultures of hepatocytes and liver non-parenchymal cells to further enhance the stable expression of liver functions. Hetero-spheroids consisting of hepatocytes and non-parenchymal cells were also formed by adding the polymer to the culture medium. The expression levels of liver functions depended on the mixing ratio of the cells. The values of albumin secretion, ammonia removal and urea synthesis were the highest when equal number of hepatocytes and non-parenchymal cells were mixed. The hetero-spheroids maintained a higher level of albumin secretion for over 4 weeks compared with co-cultured cells without the polymer and spheroids consisting of hepatocytes solely. Other liver functions such as ammonia removal and cytochrome P-450 activity in hetero-spheroid cultures were also maintained at a high level throughout the 4-week culture period.
UR - http://www.scopus.com/inward/record.url?scp=0034894421&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034894421&partnerID=8YFLogxK
U2 - 10.1016/S1369-703X(01)00095-X
DO - 10.1016/S1369-703X(01)00095-X
M3 - Article
VL - 8
SP - 135
EP - 143
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
IS - 2
ER -