Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound ran

T. Ohba, M. Nakamura, H. Nishitani, T. Nishimoto

Research output: Contribution to journalArticlepeer-review

239 Citations (Scopus)

Abstract

The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1- depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.

Original languageEnglish
Pages (from-to)1356-1358
Number of pages3
JournalScience
Volume284
Issue number5418
DOIs
Publication statusPublished - May 21 1999

All Science Journal Classification (ASJC) codes

  • General

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