TY - JOUR
T1 - Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound ran
AU - Ohba, T.
AU - Nakamura, M.
AU - Nishitani, H.
AU - Nishimoto, T.
PY - 1999/5/21
Y1 - 1999/5/21
N2 - The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1- depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.
AB - The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1- depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.
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U2 - 10.1126/science.284.5418.1356
DO - 10.1126/science.284.5418.1356
M3 - Article
C2 - 10334990
AN - SCOPUS:0033591386
VL - 284
SP - 1356
EP - 1358
JO - Science
JF - Science
SN - 0036-8075
IS - 5418
ER -