Self-regulated left-right asymmetric expression of Pitx2c in the developing mouse limb

Hidetaka Shiratori, Kenta Yashiro, Naomi Iwai, Shinya Oki, Katsura Minegishi, Yayoi Ikawa, Kohei Kanata, Hiroshi Hamada

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The transcription factor Pitx2c is expressed in primordial visceral organs in a left-right (L-R) asymmetric manner and executes situs-specific morphogenesis. Here we show that Pitx2c is also L-R asymmetrically expressed in the developing mouse limb. Human PITX2c exhibits the same transcriptional activity in the mouse limb. The asymmetric expression of Pitx2c in the limb also exhibits dorsal-ventral and anterior-posterior polarities, being confined to the posterior-dorsal region of the left limb. Left-sided Pitx2c expression in the limb is regulated by Nodal signaling through a Nodal-responsive enhancer. Pitx2c is expressed in lateral plate mesoderm (LPM)-derived cells in the left limb that contribute to various limb connective tissues. The number of Pitx2c+ cells in the left limb was found to be negatively regulated by Pitx2c itself. Although obvious defects were not apparent in the limb of mice lacking asymmetric Pitx2c expression, Pitx2c may regulate functional L-R asymmetry of the limb.

Original languageEnglish
Pages (from-to)331-341
Number of pages11
JournalDevelopmental Biology
Volume395
Issue number2
DOIs
Publication statusPublished - Nov 15 2014

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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