Self-repair protects microtubules from destruction by molecular motors

Sarah Triclin, Daisuke Inoue, Jérémie Gaillard, Zaw Min Htet, Morgan E. DeSantis, Didier Portran, Emmanuel Derivery, Charlotte Aumeier, Laura Schaedel, Karin John, Christophe Leterrier, Samara L. Reck-Peterson, Laurent Blanchoin, Manuel Théry

Research output: Contribution to journalArticlepeer-review

Abstract

Microtubule instability stems from the low energy of tubulin dimer interactions, which sets the growing polymer close to its disassembly conditions. Molecular motors use ATP hydrolysis to produce mechanical work and move on microtubules. This raises the possibility that the mechanical work produced by walking motors can break dimer interactions and trigger microtubule disassembly. We tested this hypothesis by studying the interplay between microtubules and moving molecular motors in vitro. Our results show that molecular motors can remove tubulin dimers from the lattice and rapidly destroy microtubules. We also found that dimer removal by motors was compensated for by the insertion of free tubulin dimers into the microtubule lattice. This self-repair mechanism allows microtubules to survive the damage induced by molecular motors as they move along their tracks. Our study reveals the existence of coupling between the motion of molecular motors and the renewal of the microtubule lattice.

Original languageEnglish
JournalNature Materials
DOIs
Publication statusAccepted/In press - 2021

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Fingerprint Dive into the research topics of 'Self-repair protects microtubules from destruction by molecular motors'. Together they form a unique fingerprint.

Cite this