Semaphorin 3 C drives epithelial-to-mesenchymal transition, invasiveness, and stem-like characteristics in prostate cells

Kevin J. Tam, Daniel H.F. Hui, Wilson W. Lee, Mingshu Dong, Tabitha Tombe, Ivy Z.F. Jiao, Shahram Khosravi, Ario Takeuchi, James W. Peacock, Larissa Ivanova, Igor Moskalev, Martin E. Gleave, Ralph Buttyan, Michael E. Cox, Christopher J. Ong

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Prostate cancer (PCa) is among the most commonly-occurring cancers worldwide and a leader in cancer-related deaths. Local non-invasive PCa is highly treatable but limited treatment options exist for those with locally-advanced and metastatic forms of the disease underscoring the need to identify mechanisms mediating PCa progression. The semaphorins are a large grouping of membrane-associated or secreted signalling proteins whose normal roles reside in embryogenesis and neuronal development. In this context, semaphorins help establish chemotactic gradients and direct cell movement. Various semaphorin family members have been found to be up-and down-regulated in a number of cancers. One family member, Semaphorin 3 C (SEMA3C), has been implicated in prostate, breast, ovarian, gastric, lung, and pancreatic cancer as well as glioblastoma. Given SEMA3C's roles in development and its augmented expression in PCa, we hypothesized that SEMA3C promotes epithelial-to-mesenchymal transition (EMT) and stem-like phenotypes in prostate cells. In the present study we show that ectopic expression of SEMA3C in RWPE-1 promotes the upregulation of EMT and stem markers, heightened sphere-formation, and cell plasticity. In addition, we show that SEMA3C promotes migration and invasion in vitro and cell dissemination in vivo.

Original languageEnglish
Article number11501
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017
Externally publishedYes

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Semaphorins
Epithelial-Mesenchymal Transition
Prostate
Prostatic Neoplasms
Neoplasms
Glioblastoma
Drive
Pancreatic Neoplasms
Ovarian Neoplasms
Stomach Neoplasms
Embryonic Development
Cell Movement
Lung Neoplasms
Up-Regulation
Breast Neoplasms
Phenotype
Membranes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Semaphorin 3 C drives epithelial-to-mesenchymal transition, invasiveness, and stem-like characteristics in prostate cells. / Tam, Kevin J.; Hui, Daniel H.F.; Lee, Wilson W.; Dong, Mingshu; Tombe, Tabitha; Jiao, Ivy Z.F.; Khosravi, Shahram; Takeuchi, Ario; Peacock, James W.; Ivanova, Larissa; Moskalev, Igor; Gleave, Martin E.; Buttyan, Ralph; Cox, Michael E.; Ong, Christopher J.

In: Scientific reports, Vol. 7, No. 1, 11501, 01.12.2017.

Research output: Contribution to journalArticle

Tam, KJ, Hui, DHF, Lee, WW, Dong, M, Tombe, T, Jiao, IZF, Khosravi, S, Takeuchi, A, Peacock, JW, Ivanova, L, Moskalev, I, Gleave, ME, Buttyan, R, Cox, ME & Ong, CJ 2017, 'Semaphorin 3 C drives epithelial-to-mesenchymal transition, invasiveness, and stem-like characteristics in prostate cells', Scientific reports, vol. 7, no. 1, 11501. https://doi.org/10.1038/s41598-017-11914-6
Tam, Kevin J. ; Hui, Daniel H.F. ; Lee, Wilson W. ; Dong, Mingshu ; Tombe, Tabitha ; Jiao, Ivy Z.F. ; Khosravi, Shahram ; Takeuchi, Ario ; Peacock, James W. ; Ivanova, Larissa ; Moskalev, Igor ; Gleave, Martin E. ; Buttyan, Ralph ; Cox, Michael E. ; Ong, Christopher J. / Semaphorin 3 C drives epithelial-to-mesenchymal transition, invasiveness, and stem-like characteristics in prostate cells. In: Scientific reports. 2017 ; Vol. 7, No. 1.
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