Semi-hydrolysate of paper pulp without pretreatment enables a consolidated fermentation system with in situ product recovery for the production of butanol

Tao Zhao, Kento Yasuda, Yukihiro Tashiro, Rizki Fitria Darmayanti, Kenji Sakai, Kenji Sonomoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Utilization of lignocellulosic biomasses for biobutanol fermentation usually requires costly processes of pretreatment and enzymatic hydrolysis. In this study, paper pulp (93.2% glucan) was used as a starting biomass material to produce biobutanol. We conducted enzymatic semi-hydrolysis of paper pulp without pretreatment and with low enzyme loading, which produced high concentrations of cellobiose (13.9 g L−1) and glucose (21.3 g L−1). In addition, efficient fermentation of the semi-hydrolysate was achieved similar to that with the use of commercial sugars without inhibitors. Finally, we designed a novel non-isothermal simultaneous saccharification and fermentation with in situ butanol recovery, which was composed of a repeated semi-hydrolysis process and successive butanol-extractive fermentation process under the respective optimal conditions. The consolidated system improved butanol production, butanol yields, and butanol productivities and enabled repeated use of medium when compared with other integrated hydrolysis and fermentation processes.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalBioresource Technology
Volume278
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Butanols
Butenes
Fermentation
fermentation
Pulp
hydrolysis
Recovery
Hydrolysis
Biomass
Cellobiose
Saccharification
Enzymatic hydrolysis
Glucans
biomass
Sugars
Glucose
inhibitor
sugar
glucose
Enzymes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Semi-hydrolysate of paper pulp without pretreatment enables a consolidated fermentation system with in situ product recovery for the production of butanol. / Zhao, Tao; Yasuda, Kento; Tashiro, Yukihiro; Darmayanti, Rizki Fitria; Sakai, Kenji; Sonomoto, Kenji.

In: Bioresource Technology, Vol. 278, 01.04.2019, p. 57-65.

Research output: Contribution to journalArticle

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