Semi-hydrolysis with low enzyme loading leads to highly effective butanol fermentation

Tao Zhao, Yukihiro Tashiro, Jin Zheng, Kenji Sakai, Kenji Sonomoto

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


To improve butanol fermentation efficiencies, semi-hydrolysate with low enzyme loading using H2SO4 pretreated rice straw was designed, which preferably produced cellobiose with xylose (instead of glucose). Fermentation of semi-hydrolysates avoided carbon catabolite repression (CCR) and produced higher butanol yield to enzyme loading (0.0290 g U−1), a newly proposed parameter, than the conventional glucose-oriented hydrolysate (0.00197 g U−1). Further, overall butanol productivity was improved from 0.0628 g L−1 h−1 to 0.265 g L−1 h−1 during fermentation of undetoxified semi-hydrolysate by using high cell density. A novel simultaneously repeated hydrolysis and fermentation (SRHF) was constructed by recycling of enzymes and cells, which further improved butanol yield to enzyme loading by 183% and overall butanol productivity by 6.04%. Thus, semi-hydrolysate with SRHF is a smartly designed biomass for efficient butanol fermentation of lignocellulosic materials.

Original languageEnglish
Pages (from-to)335-342
Number of pages8
JournalBioresource Technology
Publication statusPublished - Sep 2018

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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