Semi-wet peptide/protein array using supramolecular hydrogel

Shigeki Kiyonaka, Kazuki Sada, Ibuki Yoshimura, Seiji Shinkai, Nobuo Kato, Itaru Hamachi

Research output: Contribution to journalArticle

492 Citations (Scopus)

Abstract

A new semi-wet peptide/protein gel array, in which peptides or proteins were entrapped in the active form, was constructed. The hierarchical structural characteristics of supramolecular hydrogel based on a glycosylated amino acid ester were demonstrated. The gel fibers were self-assembled and stabilized by multiple interactions such as β-sheet-like hydrogen-bonding networks, van der Walls packing and hydrophobic interactions. The spontaneous gel-formation and amphiphilic property of the hydrogel was applied to supramolecular semi-wet peptide/protein gel arrays that were compatible with enzyme activity assays and the screening of enzyme inhibitors.

Original languageEnglish
Pages (from-to)58-64
Number of pages7
JournalNature Materials
Volume3
Issue number1
DOIs
Publication statusPublished - Jan 2004

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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  • Cite this

    Kiyonaka, S., Sada, K., Yoshimura, I., Shinkai, S., Kato, N., & Hamachi, I. (2004). Semi-wet peptide/protein array using supramolecular hydrogel. Nature Materials, 3(1), 58-64. https://doi.org/10.1038/nmat1034