Semi‐preparative HPLC purification of ribosomal proteins from Bacillus stearothermophilus and sequence determination of the highly conserved protein S19

Hisashi HIRANO, Klaus ECKART, Makoto KIMURA, Brigitte WITTMANN‐LIEBOLD

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Abstract

Several proteins from the Bacillus stearothermophilus 30S ribosomal subunit which could not be isolated by conventional open‐column chromatography were purified by high‐performance liquid chromatography using a semi‐preparative reverse‐phase C4 column. Protein S19 was purified by this technique and the complete amino acid sequence determined. Protein S19 was fragmented and the peptides isolated in picomole quantities were sequenced by an improved manual 4‐N,N‐dimethylaminoazobenzene‐4′‐isothiocyanate (DABITC) technique; the presence of five consecutive C‐terminal lysines in the S19 sequence was confirmed by gas‐phase sequencing and fast‐atom‐bombardment (FAB) mass spectrometry. Protein S19 is composed of 91 amino acid residues which correspond to a molecular mass of 10428 Da. 71% of the B. stearothermophilus S19 sequence was found to be identical with the corresponding ribosomal protein from Escherichia coli [Yaguchi and Wittmann (1978), FEBS Lett. 88, 227] and both sequences can be aligned without gaps. Among the known 26 amino acid sequences of the B. stearothermophilus and E. coli ribosome such a high degree of conservation has only been observed for a few proteins, all of which are known to be involved in the protein biosynthesis process. Although a clear function has not yet been assigned to protein S19, its high sequence conservation in these two eubacteria clearly indicates an important role of this protein for the function of the ribosome.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalEuropean Journal of Biochemistry
Volume170
Issue number1-2
DOIs
Publication statusPublished - Dec 1987

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Geobacillus stearothermophilus
Ribosomal Proteins
Bacilli
Purification
Sequence Analysis
High Pressure Liquid Chromatography
Proteins
Ribosomes
Amino Acid Sequence
Amino Acids
Escherichia coli
Conservation
Ribosome Subunits
Protein Biosynthesis
Biosynthesis
Liquid chromatography
Liquid Chromatography
Molecular mass
Lysine
Chromatography

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Semi‐preparative HPLC purification of ribosomal proteins from Bacillus stearothermophilus and sequence determination of the highly conserved protein S19. / HIRANO, Hisashi; ECKART, Klaus; KIMURA, Makoto; WITTMANN‐LIEBOLD, Brigitte.

In: European Journal of Biochemistry, Vol. 170, No. 1-2, 12.1987, p. 149-157.

Research output: Contribution to journalArticle

HIRANO, Hisashi ; ECKART, Klaus ; KIMURA, Makoto ; WITTMANN‐LIEBOLD, Brigitte. / Semi‐preparative HPLC purification of ribosomal proteins from Bacillus stearothermophilus and sequence determination of the highly conserved protein S19. In: European Journal of Biochemistry. 1987 ; Vol. 170, No. 1-2. pp. 149-157.
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