Hairy roots of Senna alata transformed with Agrobacterium rhizogenes, strain ATCC 15834 were induced and grown in half-strength Murashige and Skoog (MS) medium. Effects of sucrose contents and hormones on the growth and sennosides A, B production were investigated. Hairy roots cultured on hormone-free half-strength MS medium containing 5% sucrose under dark condition mostly stimulated the growth of hairy roots and increased the content of sennosides A and B yielding (169 ± 4) and (34 ± 3) μg g -1 dry wt, respectively.
|Number of pages||5|
|Journal||Zeitschrift fur Naturforschung - Section C Journal of Biosciences|
|Publication status||Published - Jan 1 2006|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)