TY - JOUR
T1 - Sense and antisense modification of glial αB-crystallin production results in alterations of stress fiber formation and thermoresistance
AU - Iwaki, Toru
AU - Iwaki, Akiko
AU - Tateishi, Jun
AU - Goldman, James E.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1994/6
Y1 - 1994/6
N2 - The phenotypic effects of selectively altering the levels of αB- crystallin in cultured glial cells were analyzed using sense and antisense approaches. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat αB-crystallin sense cDNA or an antisense cDNA regulated by a Rous sarcoma virus promoter to alter cellular levels of αB- crystallin. The antisense strategy resulted in decreased αB-crystallin levels, as revealed by Western blot and immunocytochemical analyses. The reduced αB-crystallin expression was accompanied by alterations in cellular phenotype: (a) a reduction of cell size and/or a slender cell morphology; (b) a disorganized microfilament network; and (c) a reduction of cell adhesiveness. Like HSP27, the presence of additional αB-crystallin protein confers a thermoresistant phenotype to stable transfectants. Thus, αB- crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization.
AB - The phenotypic effects of selectively altering the levels of αB- crystallin in cultured glial cells were analyzed using sense and antisense approaches. Rat C6 glioma cells and human U-373MG glioma cells were transfected with a rat αB-crystallin sense cDNA or an antisense cDNA regulated by a Rous sarcoma virus promoter to alter cellular levels of αB- crystallin. The antisense strategy resulted in decreased αB-crystallin levels, as revealed by Western blot and immunocytochemical analyses. The reduced αB-crystallin expression was accompanied by alterations in cellular phenotype: (a) a reduction of cell size and/or a slender cell morphology; (b) a disorganized microfilament network; and (c) a reduction of cell adhesiveness. Like HSP27, the presence of additional αB-crystallin protein confers a thermoresistant phenotype to stable transfectants. Thus, αB- crystallin in glioma cells plays a role in their thermal resistance and may contribute to the stability of cytoskeletal organization.
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U2 - 10.1083/jcb.125.6.1385
DO - 10.1083/jcb.125.6.1385
M3 - Article
C2 - 8207065
AN - SCOPUS:0028365799
VL - 125
SP - 1385
EP - 1393
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 6
ER -