Sensing of oligopeptides using localized surface plasmon resonances combined with Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Yuki Nakamura, Yukiko Tsuru, Masanori Fujii, Yumi Taga, Ayaka Kiya, Naotoshi Nakashima, Yasuro Niidome

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    Gold nanorods were fixed on an ITO plate and used for the spectroscopic sensing and Surface-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (SALDI-MS) of oligopeptides (angiotensin I). The longitudinal surface plasmon bands of the gold nanorods responded to the 10-10 M angiotensin solution that was cast on the ITO plate. The SALDI-MS measurements had an ultra-high sensitivity to the angiotensin on the ITO plate. A very small surface density (5 × 10-19 mol cm-2) of angiotensin could be detected at m/z = 1297 with a good signal/noise ratio (S/N = 11). The ITO plate, which was modified with gold nanorods, was found to be effective in collecting angiotensin molecules adjacent to the gold nanorods, and the SALDI processes that were induced by the photoabsorption of the gold nanorods efficiently contributed to the desorption and ionization of the angiotensin.

    Original languageEnglish
    Pages (from-to)3793-3798
    Number of pages6
    JournalNanoscale
    Volume3
    Issue number9
    DOIs
    Publication statusPublished - Sep 2011

    All Science Journal Classification (ASJC) codes

    • Materials Science(all)

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