Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement

Michihiko Nakano; Hiromichi Kasahara; Zhenhao Ding; Junya Suehiro

doi:10.1109/ICSENS.2015.7370216

Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement

Michihiko Nakano, Hiromichi Kasahara, Zhenhao Ding, Junya Suehiro

Applied Energy Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.

Original language	English
Title of host publication	2015 IEEE SENSORS - Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781479982028
DOIs	https://doi.org/10.1109/ICSENS.2015.7370216
Publication status	Published - Dec 31 2015
Event	14th IEEE SENSORS - Busan, Korea, Republic of Duration: Nov 1 2015 → Nov 4 2015

Publication series

Name	2015 IEEE SENSORS - Proceedings

Other

Other	14th IEEE SENSORS
Country/Territory	Korea, Republic of
City	Busan
Period	11/1/15 → 11/4/15

All Science Journal Classification (ASJC) codes

Instrumentation
Electronic, Optical and Magnetic Materials
Spectroscopy
Electrical and Electronic Engineering

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10.1109/ICSENS.2015.7370216

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Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. / Nakano, Michihiko; Kasahara, Hiromichi; Ding, Zhenhao et al.

2015 IEEE SENSORS - Proceedings. Institute of Electrical and Electronics Engineers Inc., 2015. 7370216 (2015 IEEE SENSORS - Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nakano, M, Kasahara, H, Ding, Z & Suehiro, J 2015, Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement. in 2015 IEEE SENSORS - Proceedings., 7370216, 2015 IEEE SENSORS - Proceedings, Institute of Electrical and Electronics Engineers Inc., 14th IEEE SENSORS, Busan, Korea, Republic of, 11/1/15. https://doi.org/10.1109/ICSENS.2015.7370216

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title = "Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement",

abstract = "We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.",

author = "Michihiko Nakano and Hiromichi Kasahara and Zhenhao Ding and Junya Suehiro",

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AU - Nakano, Michihiko

AU - Kasahara, Hiromichi

AU - Ding, Zhenhao

AU - Suehiro, Junya

PY - 2015/12/31

Y1 - 2015/12/31

N2 - We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.

AB - We proposed that a rapid detection method of DNA amplified by polymerase chain reaction (PCR) using dielectrophoresis (DEP) of microbeads. This electrical method measures the impedance change of the DEP trapped microbeads on which target DNA molecules are chemically attached. The method realizes easy and rapid DNA detection after conventional PCR. In this study, it was demonstrated that quantitative and high sensitivity detection of virus genome using the method as comparing with real-time PCR. Real-time PCR detects target DNA sensitive and quantitative by real-time monitoring of DNA amplification. As the results, the DNA detection method using microbeads DEP showed the same detection limit as the real-time PCR, and demonstrated the quantitative detection in the range of 101 to 105 copies/reaction. Our new method can realize inexpensive and rapid PCR-based viral detection by combined with conventional PCR.

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ER -

Sensitive and quantitative DNA detection by beads-based dielectrophoretic impedance measurement

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