Sensitive and selective determination of bromisovalum by high-performance liquid chromatography/particle beam mass spectrometry

Takeaki Nagata, Keiko Kudo, Tohru Imamura, Narumi Jitsufuchi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A specific procedure using high-performance liquid chromatography/particle beam mass spectrometry (HPLC/PBMS) has facilitated determination of bromisovalum in human plasma and whole blood. Bromisovalum, a sedative and hypnotic, was effectively extracted with SepPak C18 cartridges and selectively determined by HPLC/PBMS of EI mode. An originally synthesized 2-bromohexanoylurea served as the internal standard (IS). The calibration curve was linear over the concentration range 0.5-5.0 μg/g and the lower limit of detection was 0.1 μg/g (10 ng at the time of direct injection). This method could be practically applied to detect bromisovalum in the whole blood of an autopsied individual.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalForensic Science International
Volume74
Issue number3
DOIs
Publication statusPublished - Jul 28 1995

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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