Sensitive and selective determination of bromisovalum by high-performance liquid chromatography/particle beam mass spectrometry

Takeaki Nagata; Keiko Kudo; Tohru Imamura; Narumi Jitsufuchi

doi:10.1016/0379-0738(95)01762-8

Sensitive and selective determination of bromisovalum by high-performance liquid chromatography/particle beam mass spectrometry

Takeaki Nagata, Keiko Kudo, Tohru Imamura, Narumi Jitsufuchi

Social Medicine

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

A specific procedure using high-performance liquid chromatography/particle beam mass spectrometry (HPLC/PBMS) has facilitated determination of bromisovalum in human plasma and whole blood. Bromisovalum, a sedative and hypnotic, was effectively extracted with SepPak C₁₈ cartridges and selectively determined by HPLC/PBMS of EI mode. An originally synthesized 2-bromohexanoylurea served as the internal standard (IS). The calibration curve was linear over the concentration range 0.5-5.0 μg/g and the lower limit of detection was 0.1 μg/g (10 ng at the time of direct injection). This method could be practically applied to detect bromisovalum in the whole blood of an autopsied individual.

Original language	English
Pages (from-to)	205-211
Number of pages	7
Journal	Forensic Science International
Volume	74
Issue number	3
DOIs	https://doi.org/10.1016/0379-0738(95)01762-8
Publication status	Published - Jul 28 1995

All Science Journal Classification (ASJC) codes

Pathology and Forensic Medicine

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10.1016/0379-0738(95)01762-8

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title = "Sensitive and selective determination of bromisovalum by high-performance liquid chromatography/particle beam mass spectrometry",

abstract = "A specific procedure using high-performance liquid chromatography/particle beam mass spectrometry (HPLC/PBMS) has facilitated determination of bromisovalum in human plasma and whole blood. Bromisovalum, a sedative and hypnotic, was effectively extracted with SepPak C18 cartridges and selectively determined by HPLC/PBMS of EI mode. An originally synthesized 2-bromohexanoylurea served as the internal standard (IS). The calibration curve was linear over the concentration range 0.5-5.0 μg/g and the lower limit of detection was 0.1 μg/g (10 ng at the time of direct injection). This method could be practically applied to detect bromisovalum in the whole blood of an autopsied individual.",

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T1 - Sensitive and selective determination of bromisovalum by high-performance liquid chromatography/particle beam mass spectrometry

AU - Nagata, Takeaki

AU - Kudo, Keiko

AU - Imamura, Tohru

AU - Jitsufuchi, Narumi

PY - 1995/7/28

Y1 - 1995/7/28

N2 - A specific procedure using high-performance liquid chromatography/particle beam mass spectrometry (HPLC/PBMS) has facilitated determination of bromisovalum in human plasma and whole blood. Bromisovalum, a sedative and hypnotic, was effectively extracted with SepPak C18 cartridges and selectively determined by HPLC/PBMS of EI mode. An originally synthesized 2-bromohexanoylurea served as the internal standard (IS). The calibration curve was linear over the concentration range 0.5-5.0 μg/g and the lower limit of detection was 0.1 μg/g (10 ng at the time of direct injection). This method could be practically applied to detect bromisovalum in the whole blood of an autopsied individual.

AB - A specific procedure using high-performance liquid chromatography/particle beam mass spectrometry (HPLC/PBMS) has facilitated determination of bromisovalum in human plasma and whole blood. Bromisovalum, a sedative and hypnotic, was effectively extracted with SepPak C18 cartridges and selectively determined by HPLC/PBMS of EI mode. An originally synthesized 2-bromohexanoylurea served as the internal standard (IS). The calibration curve was linear over the concentration range 0.5-5.0 μg/g and the lower limit of detection was 0.1 μg/g (10 ng at the time of direct injection). This method could be practically applied to detect bromisovalum in the whole blood of an autopsied individual.

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Sensitive and selective determination of bromisovalum by high-performance liquid chromatography/particle beam mass spectrometry

Abstract

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