We developed a compact measurement system for surface plasmon fluorescence spectroscopy (SPFS) and a sensor surface for the detection of 2,4,6-trinitrotoluene (TNT). We employed a laser diode as a light source in the optical system for exciting fluorescence. Then, a fan-shaped beam was irradiated from the laser diode to a Au thin film using a beam expander. Fluorescence emission was captured using a photomultiplier detector. The sensor surface was modified with TNT analogues via a self-assembled monolayer. A limit of detection in the ppt range for TNT was obtained using a fluorescence-labeled anti- TNT monoclonal antibody in the SPFS system by indirect competitive assay.
|Number of pages||4|
|Journal||Proceedings of IEEE Sensors|
|Publication status||Published - Dec 12 2014|
|Event||13th IEEE SENSORS Conference, SENSORS 2014 - Valencia, Spain|
Duration: Nov 2 2014 → Nov 5 2014
All Science Journal Classification (ASJC) codes
- Electrical and Electronic Engineering