Sensitive detection of 2,4,6-trinitrotoluene by surface plasmon fluorescence spectroscopy

Satoshi Ito, Shuhei Tanaka, Rui Yatabe, Takeshi Onodera, Kiyoshi Toko

Research output: Contribution to journalConference article

Abstract

We developed a compact measurement system for surface plasmon fluorescence spectroscopy (SPFS) and a sensor surface for the detection of 2,4,6-trinitrotoluene (TNT). We employed a laser diode as a light source in the optical system for exciting fluorescence. Then, a fan-shaped beam was irradiated from the laser diode to a Au thin film using a beam expander. Fluorescence emission was captured using a photomultiplier detector. The sensor surface was modified with TNT analogues via a self-assembled monolayer. A limit of detection in the ppt range for TNT was obtained using a fluorescence-labeled anti- TNT monoclonal antibody in the SPFS system by indirect competitive assay.

Original languageEnglish
Article number6984999
Pages (from-to)325-328
Number of pages4
JournalProceedings of IEEE Sensors
Volume2014-December
Issue numberDecember
DOIs
Publication statusPublished - Dec 12 2014
Event13th IEEE SENSORS Conference, SENSORS 2014 - Valencia, Spain
Duration: Nov 2 2014Nov 5 2014

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Fluorescence spectroscopy
Fluorescence
Semiconductor lasers
Monoclonal antibodies
Photomultipliers
Sensors
Self assembled monolayers
Optical systems
Fans
Light sources
Assays
Detectors
Thin films

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Cite this

Sensitive detection of 2,4,6-trinitrotoluene by surface plasmon fluorescence spectroscopy. / Ito, Satoshi; Tanaka, Shuhei; Yatabe, Rui; Onodera, Takeshi; Toko, Kiyoshi.

In: Proceedings of IEEE Sensors, Vol. 2014-December, No. December, 6984999, 12.12.2014, p. 325-328.

Research output: Contribution to journalConference article

Ito, Satoshi ; Tanaka, Shuhei ; Yatabe, Rui ; Onodera, Takeshi ; Toko, Kiyoshi. / Sensitive detection of 2,4,6-trinitrotoluene by surface plasmon fluorescence spectroscopy. In: Proceedings of IEEE Sensors. 2014 ; Vol. 2014-December, No. December. pp. 325-328.
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