Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic acid polyclonal antibodies

Shingo Nakamura, Rui Yatabe, Takeshi Onodera, Kiyoshi Toko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Recently, highly functional biosensors have been developed in preparation for possible large-scale terrorist attacks using chemical warfare agents. Practically applicable sensors are required to have various abilities, such as high portability and operability, the capability of performing rapid and continuous measurement, as well as high sensitivity and selectivity. We developed the detection method of capsaicinoids, the main component of some lachrymators, using a surface plasmon resonance (SPR) immunosensor as an on-site detection sensor. Homovanillic acid, which has a vanillyl group similar to capsaicinoids such as capsaicin and dihydrocapsaicin, was bound to Concholepas concholepas hemocyanin (CCH) for use as an immunogen to generate polyclonal antibodies. An indirect competitive assay was carried out to detect capsaicinoids using SPR sensor chips on which different capsaicin analogues were immobilized. For the sensor chip on which 4-hydroxy-3-methoxybenzylamine hydrochloride was immobilized, a detection limit of 150 ppb was achieved. We found that the incubation time was not required and the detection can be completed in five minutes.

Original languageEnglish
Pages (from-to)374-384
Number of pages11
JournalBiosensors
Volume3
Issue number4
DOIs
Publication statusPublished - Jan 1 2013

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Homovanillic Acid
Surface Plasmon Resonance
Capsaicin
Surface plasmon resonance
Chemical Warfare Agents
Hemocyanin
Antibodies
Sensors
Biosensing Techniques
Limit of Detection
Immunosensors
Biosensors
Assays
dihydrocapsaicin
4-hydroxy 3-methoxybenzylamine

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Sensitive detection of capsaicinoids using a surface plasmon resonance sensor with anti-homovanillic acid polyclonal antibodies. / Nakamura, Shingo; Yatabe, Rui; Onodera, Takeshi; Toko, Kiyoshi.

In: Biosensors, Vol. 3, No. 4, 01.01.2013, p. 374-384.

Research output: Contribution to journalArticle

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