Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry

X. X. Zhang, K. Kudo, T. Imamura, N. Jitsufuchi, T. Nagata

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

A reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the levels of bromazepam in human tissues. Bromazepam was extracted from body tissues using a three-step solvent extraction procedure. N-Desmethyldiazepam served as the internal standard. Selected ion monitoring with m/z 317 for bromazepam and m/z 270 or internal standard was used for quantitation. Calibration curves in all body tissues were linear over the concentration range from 50-500 ng/g. The lower detection limit in body tissues was 2-5 ng/g and the absolute recovery in body tissues was 7.8-68.0%. This method was used to determine the levels of bromazepam in tissues of an autopsied individual who had been prescribed psychotropic drugs and who was found dead in a car.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume677
Issue number1
DOIs
Publication statusPublished - Feb 23 1996

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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