Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry

X. X. Zhang, Keiko Kudo, T. Imamura, N. Jitsufuchi, T. Nagata

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the levels of bromazepam in human tissues. Bromazepam was extracted from body tissues using a three-step solvent extraction procedure. N-Desmethyldiazepam served as the internal standard. Selected ion monitoring with m/z 317 for bromazepam and m/z 270 or internal standard was used for quantitation. Calibration curves in all body tissues were linear over the concentration range from 50-500 ng/g. The lower detection limit in body tissues was 2-5 ng/g and the absolute recovery in body tissues was 7.8-68.0%. This method was used to determine the levels of bromazepam in tissues of an autopsied individual who had been prescribed psychotropic drugs and who was found dead in a car.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume677
Issue number1
DOIs
Publication statusPublished - Feb 23 1996

Fingerprint

Bromazepam
Gas chromatography
Mass spectrometry
Tissue
Nordazepam
Psychotropic Drugs
Solvent extraction
Railroad cars
Gases
Calibration
Ions
Recovery
Monitoring

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry. / Zhang, X. X.; Kudo, Keiko; Imamura, T.; Jitsufuchi, N.; Nagata, T.

In: Journal of Chromatography B: Biomedical Applications, Vol. 677, No. 1, 23.02.1996, p. 111-116.

Research output: Contribution to journalArticle

Zhang, X. X. ; Kudo, Keiko ; Imamura, T. ; Jitsufuchi, N. ; Nagata, T. / Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry. In: Journal of Chromatography B: Biomedical Applications. 1996 ; Vol. 677, No. 1. pp. 111-116.
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