Sensitive determination of D-amino acids in mammals and the effect of D-amino-acid oxidase activity on their amounts

Kenji Hamase, Ryuichi Konno, Akiko Morikawa, Kiyoshi Zaitsu

Research output: Contribution to journalReview article

64 Citations (Scopus)


The determination of small amounts of D-amino acids in mammalian tissues is still a challenging theme in the separation sciences. In this review, various gas-chromatographic and high-performance liquid chromatographic methods are discussed including highly selective and sensitive column-switching procedures. Based on these methods, the distributions of D-aspartic acid, D-serine, D-alanine, D-leucine and D-proline have been clarified in the mouse brain. As the regulation mechanisms of D-amino acid amounts in mammals, we focused on the D-amino-acid oxidase, which catalyzes the degradation of D-amino acids. Using the mutant mouse strain lacking D-amino-acid oxidase activity, the effects of the enzymatic activity on the amounts and distributions of various D-amino acids have been investigated.

Original languageEnglish
Pages (from-to)1578-1584
Number of pages7
JournalBiological and Pharmaceutical Bulletin
Issue number9
Publication statusPublished - Sep 1 2005


All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science

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