Sensitive determination of diazepam and N-desmethyldiazepam in human material using capillary gas chromatography-mass spectrometry

Keiko Kudo, T. Nagata, K. Kimura, T. Imamura, Mami Noda

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A reliable and sensitive capillary gas chromatographic-mass spectrometric method was developed for the detection and determination of diazepam and its major metabolite, N-desmethyldiazepam, in human material. Medazepam served as the internal standard. Quantitative determination was achieved using mass fragmentography with selected ions of m/z 256 for diazepam and m/z 242 for N-desmethyldiazepam and medazepam. The limit of detection was 1 ng/g and the recoveries were 98.54±3.95% for diazepam and 98.66±6.48% for N-desmethyldiazepam. The calibration graph was linear over the concentration range from 1.0 ng/g to 1.0 μg/g for diazepam and N-desmethyldiazepam. Using this method, trace amounts of diazepam and N-desmethyldiazepam were detected in the tissues of an autopsied individual.

Original languageEnglish
Pages (from-to)353-359
Number of pages7
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume431
Issue numberC
DOIs
Publication statusPublished - Jan 1 1988

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Nordazepam
Diazepam
Gas chromatography
Mass spectrometry
Medazepam
Metabolites
Gases
Calibration
Ions
Tissue
Recovery

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Sensitive determination of diazepam and N-desmethyldiazepam in human material using capillary gas chromatography-mass spectrometry. / Kudo, Keiko; Nagata, T.; Kimura, K.; Imamura, T.; Noda, Mami.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 431, No. C, 01.01.1988, p. 353-359.

Research output: Contribution to journalArticle

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