TY - JOUR
T1 - Sensitive determination of diazepam and N-desmethyldiazepam in human material using capillary gas chromatography-mass spectrometry
AU - Kudo, K.
AU - Nagata, T.
AU - Kimura, K.
AU - Imamura, T.
AU - Noda, M.
N1 - Funding Information:
We thank M. Ohara for comments and suggestions. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 63770386) from the Japanese Ministry of Education.
PY - 1988
Y1 - 1988
N2 - A reliable and sensitive capillary gas chromatographic-mass spectrometric method was developed for the detection and determination of diazepam and its major metabolite, N-desmethyldiazepam, in human material. Medazepam served as the internal standard. Quantitative determination was achieved using mass fragmentography with selected ions of m/z 256 for diazepam and m/z 242 for N-desmethyldiazepam and medazepam. The limit of detection was 1 ng/g and the recoveries were 98.54±3.95% for diazepam and 98.66±6.48% for N-desmethyldiazepam. The calibration graph was linear over the concentration range from 1.0 ng/g to 1.0 μg/g for diazepam and N-desmethyldiazepam. Using this method, trace amounts of diazepam and N-desmethyldiazepam were detected in the tissues of an autopsied individual.
AB - A reliable and sensitive capillary gas chromatographic-mass spectrometric method was developed for the detection and determination of diazepam and its major metabolite, N-desmethyldiazepam, in human material. Medazepam served as the internal standard. Quantitative determination was achieved using mass fragmentography with selected ions of m/z 256 for diazepam and m/z 242 for N-desmethyldiazepam and medazepam. The limit of detection was 1 ng/g and the recoveries were 98.54±3.95% for diazepam and 98.66±6.48% for N-desmethyldiazepam. The calibration graph was linear over the concentration range from 1.0 ng/g to 1.0 μg/g for diazepam and N-desmethyldiazepam. Using this method, trace amounts of diazepam and N-desmethyldiazepam were detected in the tissues of an autopsied individual.
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U2 - 10.1016/S0378-4347(00)83103-5
DO - 10.1016/S0378-4347(00)83103-5
M3 - Article
C2 - 3149641
AN - SCOPUS:0023677397
SN - 0378-4347
VL - 431
SP - 353
EP - 359
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
IS - C
ER -