Sensitive determination of sulpiride in human plasma by high-performance liquid chromatography

H. Tokunaga, K. Kudo, N. Jitsufuchi, Y. Ohtsuka, T. Imamura

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Abstract

We developed a simple, sensitive and reliable method for the determination of sulpiride, a specific antipsychotic drug, in human plasma using high-performance liquid chromatography. A structurally related benzamide, tiapride, was used as the internal standard. A Sep-Pak C18 cartridge was used to extract a sample from 1 ml of plasma. The extract was dissolved in methylene chloride, and then back-extracted with 0.01 M hydrochloric acid. The aqueous layer was put on a octadecylsilica column with a mobile phase of 50% acetonitrile in 0.01 M phosphate buffer (pH 3.0). A fluorescence detector with excitation at 300 nm and emission at 365 nm was used for detection. The calibration curve was linear in the concentration range of 10-1500 ng/ml, and the lower limit of detection was 1 ng/ml. We used this method to examine plasma levels of sulpiride in 14 inpatients being treated with sulpiride for 6 months. The determined plasma levels were 70.1-1121.2 ng/ml, and the correlation between daily dose and plasma concentration was positive. This simple, reliable method is expected to be put to good use in forensic and hospital laboratories.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume691
Issue number1
DOIs
Publication statusPublished - Mar 28 1997

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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