Abstract
We developed a simple, sensitive and reliable method for the determination of sulpiride, a specific antipsychotic drug, in human plasma using high-performance liquid chromatography. A structurally related benzamide, tiapride, was used as the internal standard. A Sep-Pak C18 cartridge was used to extract a sample from 1 ml of plasma. The extract was dissolved in methylene chloride, and then back-extracted with 0.01 M hydrochloric acid. The aqueous layer was put on a octadecylsilica column with a mobile phase of 50% acetonitrile in 0.01 M phosphate buffer (pH 3.0). A fluorescence detector with excitation at 300 nm and emission at 365 nm was used for detection. The calibration curve was linear in the concentration range of 10-1500 ng/ml, and the lower limit of detection was 1 ng/ml. We used this method to examine plasma levels of sulpiride in 14 inpatients being treated with sulpiride for 6 months. The determined plasma levels were 70.1-1121.2 ng/ml, and the correlation between daily dose and plasma concentration was positive. This simple, reliable method is expected to be put to good use in forensic and hospital laboratories.
| Original language | English |
|---|---|
| Pages (from-to) | 203-207 |
| Number of pages | 5 |
| Journal | Journal of Chromatography B: Biomedical Applications |
| Volume | 691 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Mar 28 1997 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
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Sensitive determination of sulpiride in human plasma by high-performance liquid chromatography. / Tokunaga, H.; Kudo, Keiko; Jitsufuchi, N.; Ohtsuka, Y.; Imamura, T.
In: Journal of Chromatography B: Biomedical Applications, Vol. 691, No. 1, 28.03.1997, p. 203-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sensitive determination of sulpiride in human plasma by high-performance liquid chromatography
AU - Tokunaga, H.
AU - Kudo, Keiko
AU - Jitsufuchi, N.
AU - Ohtsuka, Y.
AU - Imamura, T.
PY - 1997/3/28
Y1 - 1997/3/28
N2 - We developed a simple, sensitive and reliable method for the determination of sulpiride, a specific antipsychotic drug, in human plasma using high-performance liquid chromatography. A structurally related benzamide, tiapride, was used as the internal standard. A Sep-Pak C18 cartridge was used to extract a sample from 1 ml of plasma. The extract was dissolved in methylene chloride, and then back-extracted with 0.01 M hydrochloric acid. The aqueous layer was put on a octadecylsilica column with a mobile phase of 50% acetonitrile in 0.01 M phosphate buffer (pH 3.0). A fluorescence detector with excitation at 300 nm and emission at 365 nm was used for detection. The calibration curve was linear in the concentration range of 10-1500 ng/ml, and the lower limit of detection was 1 ng/ml. We used this method to examine plasma levels of sulpiride in 14 inpatients being treated with sulpiride for 6 months. The determined plasma levels were 70.1-1121.2 ng/ml, and the correlation between daily dose and plasma concentration was positive. This simple, reliable method is expected to be put to good use in forensic and hospital laboratories.
AB - We developed a simple, sensitive and reliable method for the determination of sulpiride, a specific antipsychotic drug, in human plasma using high-performance liquid chromatography. A structurally related benzamide, tiapride, was used as the internal standard. A Sep-Pak C18 cartridge was used to extract a sample from 1 ml of plasma. The extract was dissolved in methylene chloride, and then back-extracted with 0.01 M hydrochloric acid. The aqueous layer was put on a octadecylsilica column with a mobile phase of 50% acetonitrile in 0.01 M phosphate buffer (pH 3.0). A fluorescence detector with excitation at 300 nm and emission at 365 nm was used for detection. The calibration curve was linear in the concentration range of 10-1500 ng/ml, and the lower limit of detection was 1 ng/ml. We used this method to examine plasma levels of sulpiride in 14 inpatients being treated with sulpiride for 6 months. The determined plasma levels were 70.1-1121.2 ng/ml, and the correlation between daily dose and plasma concentration was positive. This simple, reliable method is expected to be put to good use in forensic and hospital laboratories.
UR - http://www.scopus.com/inward/record.url?scp=0030938465&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030938465&partnerID=8YFLogxK
U2 - 10.1016/S0378-4347(96)00428-8
DO - 10.1016/S0378-4347(96)00428-8
M3 - Article
VL - 691
SP - 203
EP - 207
JO - Journal of Chromatography B: Biomedical Sciences and Applications
JF - Journal of Chromatography B: Biomedical Sciences and Applications
SN - 0378-4347
IS - 1
ER -