Sensitive determination of ∆9-tetrahydrocannabinol in human tissues by gc-ms

Keiko Kudo, Takeaki Nagata, Kojiro Kimura, Tohru Imamura, Narumi Jitsufuchi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A reliable, simple, and sensitive method was devised to determine the levels of ∆9-tetrahydrocannabinol (THC) in human solid tissues. THC was effectively extracted with acetonitrile, and interfering compounds were removed by washing with a solution of sodium hydroxide and hydrochloric acid. THC was then derivatized by methylation and subjected to gas chromatography-mass spectrometry. Deuterated methyl THC (THC-CD3) was used as an internal standard. The calibration curves were linear in the concentration range from 1 to 100 ng/g, and the lower limit of detection was 1 ng/g in all samples examined. The accuracy and precision of the method were evaluated in every tissue sample at two different concentrations, 5 and 50 ng per sample. The coefficient of variation ranged from 3.4 to 11.6%. We used this method to identify THC in tissues from an autopsied individual, and the distribution of this drug in the tissues was evaluated based on medico-legal criteria.

Original languageEnglish
Pages (from-to)87-90
Number of pages4
JournalJournal of Analytical Toxicology
Volume19
Issue number2
DOIs
Publication statusPublished - Jan 1 1995

Fingerprint

Dronabinol
Tissue
Methylation
methylation
hydrochloric acid
Hydrochloric acid
Acetonitrile
Washing
Gas chromatography
hydroxide
Mass spectrometry
Sodium Hydroxide
gas chromatography
drug
Hydrochloric Acid
mass spectrometry
Sodium
sodium
Calibration
calibration

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

Cite this

Sensitive determination of ∆9-tetrahydrocannabinol in human tissues by gc-ms. / Kudo, Keiko; Nagata, Takeaki; Kimura, Kojiro; Imamura, Tohru; Jitsufuchi, Narumi.

In: Journal of Analytical Toxicology, Vol. 19, No. 2, 01.01.1995, p. 87-90.

Research output: Contribution to journalArticle

Kudo, K, Nagata, T, Kimura, K, Imamura, T & Jitsufuchi, N 1995, 'Sensitive determination of ∆9-tetrahydrocannabinol in human tissues by gc-ms', Journal of Analytical Toxicology, vol. 19, no. 2, pp. 87-90. https://doi.org/10.1093/jat/19.2.87
Kudo, Keiko ; Nagata, Takeaki ; Kimura, Kojiro ; Imamura, Tohru ; Jitsufuchi, Narumi. / Sensitive determination of ∆9-tetrahydrocannabinol in human tissues by gc-ms. In: Journal of Analytical Toxicology. 1995 ; Vol. 19, No. 2. pp. 87-90.
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