Sensitivity improvement of dielectrophoretic impedance measurement by bacteria concentration using negative dielectrophoresis

Research output: Contribution to journalArticlepeer-review

Abstract

Dielectrophoretic impedance measurement (DEPIM) is a method to detect bacteria in an aqueous solution. DEPIM consists of two simultaneous processes: dielectrophoretic trapping of the target bacteria onto a microelectrode and measurement of impedance change caused by trapping bacteria. In this study, a new bacterial concentration process was combined with DEPIM to improve its sensitivity. The concentration process was based on negative dielectrophoresis of bacteria caused by specific configuration of parallel electrodes. A ditched dielectric thin layer deposited on one of the parallel electrodes causes non-uniform electric field resulting in occurring negative dielectrophoresis. Two electrode configurations, one is for the bacteria concentration and the other is for DEPIM, were fabricated in a microchannel. As a result, the detection sensitivity of DEPIM increased about 1.6 times by combining the concentration.

Original languageEnglish
Pages (from-to)148-152
Number of pages5
JournalIEEJ Transactions on Sensors and Micromachines
Volume136
Issue number5
DOIs
Publication statusPublished - 2016

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering
  • Electrical and Electronic Engineering

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