Separable detection of lipophilic-and hydrophilic-phase free radicals from the ESR spectrum of nitroxyl radical in transient MCAO mice

Mayumi Yamato, Takeshi Shiba, Ken Ichi Yamada, Toshiaki Watanabe, Hideo Utsumi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Free radicals are believed to be key factors that promote ischemia reperfusion injury in the brain. This study used the characteristic spectrum of methoxycarbonyl-PROXYL to detect free radical reactions in hydrophilic and lipophilic compartments in a transient middle cerebral artery occlusion (MCAO) mouse model. Methoxycarbonyl-PROXYL, which has a high water/octanol partition coefficient, allows the detection of nitroxyl radical in both compartments simultaneously. Free radicals generation was analysed from the enhanced ESR signal decay rate of methoxycarbonyl-PROXYL. The signal decay rate in the lipidic compartment was significantly enhanced 1 h after reperfusion following MCAO. The enhanced signal decay rate was significantly suppressed by Trolox. The accumulation of lipid peroxidation products increased by 6 h post-reperfusion and was suppressed by methoxycarbonyl-PROXYL or Trolox. These results demonstrate that information pertaining to different sites of free radical generation in vivo can be obtained simultaneously and that lipid-derived radicals are generated in transient MCAO mice.

Original languageEnglish
Pages (from-to)844-851
Number of pages8
JournalFree Radical Research
Volume43
Issue number9
DOIs
Publication statusPublished - Nov 30 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry

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