Separate roles of structured and unstructured regions of Y-family DNA polymerases.

Haruo Ohmori, Tomo Hanafusa, Eiji Ohashi, Cyrus Vaziri

Research output: Contribution to journalReview articlepeer-review

35 Citations (Scopus)

Abstract

All organisms have multiple DNA polymerases specialized for translesion DNA synthesis (TLS) on damaged DNA templates. Mammalian TLS DNA polymerases include Pol eta, Pol iota, Pol kappa, and Rev1 (all classified as "Y-family" members) and Pol zeta (a "B-family" member). Y-family DNA polymerases have highly structured catalytic domains; however, some of these proteins adopt different structures when bound to DNA (such as archaeal Dpo4 and human Pol kappa), while others maintain similar structures independently of DNA binding (such as archaeal Dbh and Saccharomyces cerevisiae Pol eta). DNA binding-induced structural conversions of TLS polymerases depend on flexible regions present within the catalytic domains. In contrast, noncatalytic regions of Y-family proteins, which contain multiple domains and motifs for interactions with other proteins, are predicted to be mostly unstructured, except for short regions corresponding to ubiquitin-binding domains. In this review we discuss how the organization of structured and unstructured regions in TLS polymerases is relevant to their regulation and function during lesion bypass.

Original languageEnglish
Pages (from-to)99-146
Number of pages48
JournalAdvances in protein chemistry and structural biology
Volume78
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry

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