Separation of hydrophobic metabolites using monolithic silica column in high-performance liquid chromatography and supercritical fluid chromatography

Takeshi Bamba, Eiichiro Fukusaki

Research output: Contribution to journalReview article

12 Citations (Scopus)

Abstract

Monolithic silica columns have very low back-pressures and offer several advantages over conventional columns packed with spherical particles, such as high separation efficiency and rapid analysis. In this review, we report the applicability of monolithic silica columns for the analysis of complex hydrophobic metabolites. We have used monolithic columns in HPLC and developed a separation technique for the high-speed and high-resolution analysis of the geometric analogs of natural polyprenols. We also used monolithic columns in supercritical fluid chromatography for the successful separation of the structural isomers of carotenoids after deciding the analytical conditions that were suitable for this separation and have developed a method for profiling biological samples containing complex matrices. We have proved that excellent resolution can be obtained by connecting a number of monolithic columns in series.

Original languageEnglish
Pages (from-to)2699-2706
Number of pages8
JournalJournal of Separation Science
Volume32
Issue number15-16
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

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High performance liquid chromatography
Metabolites
Silicon Dioxide
Silica
Carotenoids
Isomers
Supercritical Fluid Chromatography

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Filtration and Separation

Cite this

Separation of hydrophobic metabolites using monolithic silica column in high-performance liquid chromatography and supercritical fluid chromatography. / Bamba, Takeshi; Fukusaki, Eiichiro.

In: Journal of Separation Science, Vol. 32, No. 15-16, 01.12.2009, p. 2699-2706.

Research output: Contribution to journalReview article

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