Separation of Lipids

T. Yamada, K. Taguchi, Takeshi Bamba

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Supercritical fluid chromatography (SFC) is one of the useful techniques used in lipid analysis. Since lipids comprise an enormous number of molecular species with similar structures, high-resolution chromatographic separation, coupled with mass spectrometry (MS) or other detection methods, is required for detailed profiling of molecular species. The high diffusivity and low polarity of supercritical carbon dioxide (SCCO2) enables the separation of individual lipid molecular species with good resolution. The low viscosity of SCCO2 allows for a high flow rate of the SFC mobile phase, resulting in shorter separation time compared with liquid chromatography (LC). In addition, polar lipids can be analyzed using gradient elution with SCCO2 and a modifier.Supercritical fluid extraction (SFE) can be combined directly with SFC, enabling high-throughput analysis of thermally labile and oxidizable hydrophobic compounds. These supercritical fluid-related techniques have potential applications in biomarker discovery and elucidation of the detailed mechanisms of lipid metabolism.

Original languageEnglish
Title of host publicationSupercritical Fluid Chromatography
Subtitle of host publicationHandbooks in Separation Science
PublisherElsevier Inc.
Pages419-438
Number of pages20
ISBN (Electronic)9780128093672
ISBN (Print)9780128092071
DOIs
Publication statusPublished - Feb 20 2017

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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    Yamada, T., Taguchi, K., & Bamba, T. (2017). Separation of Lipids. In Supercritical Fluid Chromatography: Handbooks in Separation Science (pp. 419-438). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-809207-1.00014-8