Abstract
Chlorophyllides and pheophorbides were separated by ion-suppression high-performance liquid chromatography using an octadecyl silica column eluted with 80-95% (v/v) methanol in water containing 13 mM acetic (final pH 4.2) as the suppessing ion. The separated pigments were detected fluorometrically with a sensitivity in the picomol range without artifact formation. This technique can be used not only for the determination of non-esterified chlorophylls, but also for esterified chlorophylls, thus enabling the simultaneous identification of chlorophylls and their derivatives.
| Original language | English |
|---|---|
| Pages (from-to) | 141-149 |
| Number of pages | 9 |
| Journal | Journal of Chromatography A |
| Volume | 298 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1984 |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Biochemistry
- Organic Chemistry