Separation of non-esterified chlorophyls by ion-suppression high-performance liquid chromatography

Yuzo Shioi, Michio Doi, Tsutomu Sasa

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Chlorophyllides and pheophorbides were separated by ion-suppression high-performance liquid chromatography using an octadecyl silica column eluted with 80-95% (v/v) methanol in water containing 13 mM acetic (final pH 4.2) as the suppessing ion. The separated pigments were detected fluorometrically with a sensitivity in the picomol range without artifact formation. This technique can be used not only for the determination of non-esterified chlorophylls, but also for esterified chlorophylls, thus enabling the simultaneous identification of chlorophylls and their derivatives.

Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalJournal of Chromatography A
Volume298
Issue numberC
DOIs
Publication statusPublished - 1984

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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