Separation of transmitted light and scattering components in transmitted microscopy

Mihoko Shimano, Ryoma Bise, Yinqiang Zheng, Imari Sato

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

In transmitted light microscopy, a specimen tends to be observed as unclear. This is caused by a phenomenon that an image sensor captures the sum of these scattered light rays traveled from different paths due to scattering. To cope with this problem, we propose a novel computational photography approach for separating directly transmitted light from the scattering light in a transmitted light microscope by using high-frequency lighting. We first investigated light paths and clarified what types of light overlap in transmitted light microscopy. The scattered light can be simply represented and removed by using the difference in observations between focused and unfocused conditions, where the high-frequency illumination becomes homogeneous. Our method makes a novel spatial multiple-spectral absorption analysis possible, which requires absorption coefficients to be measured in each spectrum at each position. Experiments on real biological tissues demonstrated the effectiveness of our method.

Original languageEnglish
Title of host publicationMedical Image Computing and Computer Assisted Intervention − MICCAI 2017 - 20th International Conference, Proceedings
EditorsPierre Jannin, Simon Duchesne, Maxime Descoteaux, Alfred Franz, D. Louis Collins, Lena Maier-Hein
PublisherSpringer Verlag
Pages12-20
Number of pages9
ISBN (Print)9783319661841
DOIs
Publication statusPublished - Jan 1 2017
Event20th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2017 - Quebec City, Canada
Duration: Sep 11 2017Sep 13 2017

Publication series

NameLecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics)
Volume10434 LNCS
ISSN (Print)0302-9743
ISSN (Electronic)1611-3349

Other

Other20th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2017
CountryCanada
CityQuebec City
Period9/11/179/13/17

All Science Journal Classification (ASJC) codes

  • Theoretical Computer Science
  • Computer Science(all)

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  • Cite this

    Shimano, M., Bise, R., Zheng, Y., & Sato, I. (2017). Separation of transmitted light and scattering components in transmitted microscopy. In P. Jannin, S. Duchesne, M. Descoteaux, A. Franz, D. L. Collins, & L. Maier-Hein (Eds.), Medical Image Computing and Computer Assisted Intervention − MICCAI 2017 - 20th International Conference, Proceedings (pp. 12-20). (Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics); Vol. 10434 LNCS). Springer Verlag. https://doi.org/10.1007/978-3-319-66185-8_2