Sequence of general transcription factor TFIIB and relationships to other initiation factors

Sohail Malik, Koji Hisatake, Hideki Sumimoto, Masami Horikoshi, Robert G. Roeder

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66 Citations (Scopus)

Abstract

Transcription factor TFIIB is a ubiquitous factor required for transcription initiation by RNA polymerase II. Previous studies have suggested that TFIIB serves as a bridge between the "TATA"-binding factor (TFIID) and RNA polymerase II during preinitiation complex assembly and, more recently, that TFIIB can be a target of acidic activators. We have purified TFIIB to homogeneity, shown that activity resides in a 33-kDa polypeptide, and obtained cDNAs encoding functional TFIIB. TFIIB contains a region with amino acid sequence similarity to a highly conserved region of prokaryotic σ factors. This is consistent with analogous functions for these factors in promoter recognition by RNA polymerases and with similar findings for TFIID, TFIIE, and TFIIF/RAP30. Like TFIID, TFIIB contains both a large imperfect repeat that could contribute an element of symmetry to the folded protein and clusters of basic residues that could interact with acidic activator domains. These findings argue for a common origin of TFIIB, TFIID, and other general transcription factors and for the evolutionary segregation of complementary functions.

Original languageEnglish
Pages (from-to)9553-9557
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number21
DOIs
Publication statusPublished - Jan 1 1991
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • General

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