Sequence-Selective Separation of Oligonucleotides and DNA Fragments by Using Polyethyleneglycol-Bound Intercalators

Shigeori Takenaka, Kunihiko Dohtsu, Naotoshi Nakashima, Makoto Takagi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Anthracene, acridine and quinacrine which possess base- and sequence-selectivity in their DNA-binding (intercalating) properties were covalentty bound to poly(ethyleneglycol) (PEG) via ester bonds. These PEG-bound intercalators induced the retardation of DNA restriction fragments and oligonucleotides (pentadecamers) in the electrophoresis in agarose gel and polyacrylamide gel, respectively. The pattern of mobility retardation depended on the base composition of the DNA fragments and oligonucleotides, reflecting the base selectivity of the parent intercalators. The PEG-bound intercalators also discriminated the base sequence, and the oligonucleotides of nearly sequence-isomereric structures such as (TA)7T and A8T7 were mutually readily separated.

Original languageEnglish
Pages (from-to)557-560
Number of pages4
Journalanalytical sciences
Volume3
Issue number6
DOIs
Publication statusPublished - Jan 1 1987

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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