Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice

Takuya Takahashi, Hitomi Matsuzaki, Shin ichi Tomizawa, Eiichi Okamura, Tomoko Ichiyanagi, Akiyoshi Fukamizu, Hiroyuki Sasaki, Keiji Tanimoto

    Research output: Contribution to journalArticle

    Abstract

    Allele-specific methylation of the endogenous H19 imprinting control region (ICR) is established in sperm. We previously showed that the paternal H19 ICR in yeast artificial chromosome (YAC) transgenic mice (TgM) was preferentially methylated in somatic cells, but not in germ cells, suggesting that differential methylation could be established after fertilization. In this report, we discovered small RNA molecules in growing oocytes, the nucleotide sequences of which mapped to the H19 ICR. To test if these small RNA sequences play a role in the establishment of differential methylation, we deleted the sequences from the H19 ICR DNA and generated YAC TgM. In somatic cells of these mice, methylation imprinting of the transgene was normally established. In addition, the mutant fragment was not methylated in sperm and eggs. These data demonstrate that sequences in the H19 ICR that correspond to the small RNA sequences are dispensable for methylation imprinting in YAC TgM.

    Original languageEnglish
    Pages (from-to)26-34
    Number of pages9
    JournalGene
    Volume508
    Issue number1
    DOIs
    Publication statusPublished - Oct 15 2012

    Fingerprint

    Yeast Artificial Chromosomes
    Methylation
    Transgenic Mice
    Oocytes
    RNA
    Spermatozoa
    Transgenes
    Fertilization
    Germ Cells
    Eggs
    Alleles
    DNA

    All Science Journal Classification (ASJC) codes

    • Genetics

    Cite this

    Takahashi, T., Matsuzaki, H., Tomizawa, S. I., Okamura, E., Ichiyanagi, T., Fukamizu, A., ... Tanimoto, K. (2012). Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice. Gene, 508(1), 26-34. https://doi.org/10.1016/j.gene.2012.07.062

    Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice. / Takahashi, Takuya; Matsuzaki, Hitomi; Tomizawa, Shin ichi; Okamura, Eiichi; Ichiyanagi, Tomoko; Fukamizu, Akiyoshi; Sasaki, Hiroyuki; Tanimoto, Keiji.

    In: Gene, Vol. 508, No. 1, 15.10.2012, p. 26-34.

    Research output: Contribution to journalArticle

    Takahashi, T, Matsuzaki, H, Tomizawa, SI, Okamura, E, Ichiyanagi, T, Fukamizu, A, Sasaki, H & Tanimoto, K 2012, 'Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice', Gene, vol. 508, no. 1, pp. 26-34. https://doi.org/10.1016/j.gene.2012.07.062
    Takahashi, Takuya ; Matsuzaki, Hitomi ; Tomizawa, Shin ichi ; Okamura, Eiichi ; Ichiyanagi, Tomoko ; Fukamizu, Akiyoshi ; Sasaki, Hiroyuki ; Tanimoto, Keiji. / Sequences in the H19 ICR that are transcribed as small RNA in oocytes are dispensable for methylation imprinting in YAC transgenic mice. In: Gene. 2012 ; Vol. 508, No. 1. pp. 26-34.
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    abstract = "Allele-specific methylation of the endogenous H19 imprinting control region (ICR) is established in sperm. We previously showed that the paternal H19 ICR in yeast artificial chromosome (YAC) transgenic mice (TgM) was preferentially methylated in somatic cells, but not in germ cells, suggesting that differential methylation could be established after fertilization. In this report, we discovered small RNA molecules in growing oocytes, the nucleotide sequences of which mapped to the H19 ICR. To test if these small RNA sequences play a role in the establishment of differential methylation, we deleted the sequences from the H19 ICR DNA and generated YAC TgM. In somatic cells of these mice, methylation imprinting of the transgene was normally established. In addition, the mutant fragment was not methylated in sperm and eggs. These data demonstrate that sequences in the H19 ICR that correspond to the small RNA sequences are dispensable for methylation imprinting in YAC TgM.",
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    AU - Ichiyanagi, Tomoko

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