Sequencing and population analysis of four novel HLA-DPA1 alleles

Haruhito Harada, Akinori Kimura, Rui Ping Dong, Xing Pei Xu, Kuldeep Bhatia, Takehiko Sasazuki

Research output: Contribution to journalArticle

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Abstract

We determined the base sequences of the HLA-DPA1 gene from four B-lymphoblastoid cell lines (CB6B, LKT3, AMAI, and T7526) that showed distinct electrophoretic patterns of single-stranded polymerase chain reaction products of the HLA-DPA1 gene. The novel HLA-DPA1 alleles of CB6B, LKT3, AMAI, and T7526 were designated DPA1*02021, DPA1*02022, DPA1*0301, and DPA1*0401, respectively. Although there was only one base substitution between DPA1*02021 and DPA1*02022, the single-strand conformation polymorphism of these two alleles was clearly demonstrated by electrophoresis in a nondenaturing polyacrylamide gel containing 10% glycerol. In addition, we genotyped for the HLA-DPA1 gene of healthy unrelated Oriental individuals-i.e., 227 Japanese, 88 Papua New Guineans, and 41 Buyi-Chinese-to demonstrate the ethnic distribution of the HLA-DPA1 alleles.

Original languageEnglish
Pages (from-to)173-178
Number of pages6
JournalHuman Immunology
Volume35
Issue number3
DOIs
Publication statusPublished - Jan 1 1992

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Alleles
Population
Genes
Glycerol
Electrophoresis
HLA-DPA1 antigen
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Harada, H., Kimura, A., Dong, R. P., Xu, X. P., Bhatia, K., & Sasazuki, T. (1992). Sequencing and population analysis of four novel HLA-DPA1 alleles. Human Immunology, 35(3), 173-178. https://doi.org/10.1016/0198-8859(92)90102-S

Sequencing and population analysis of four novel HLA-DPA1 alleles. / Harada, Haruhito; Kimura, Akinori; Dong, Rui Ping; Xu, Xing Pei; Bhatia, Kuldeep; Sasazuki, Takehiko.

In: Human Immunology, Vol. 35, No. 3, 01.01.1992, p. 173-178.

Research output: Contribution to journalArticle

Harada, H, Kimura, A, Dong, RP, Xu, XP, Bhatia, K & Sasazuki, T 1992, 'Sequencing and population analysis of four novel HLA-DPA1 alleles', Human Immunology, vol. 35, no. 3, pp. 173-178. https://doi.org/10.1016/0198-8859(92)90102-S
Harada H, Kimura A, Dong RP, Xu XP, Bhatia K, Sasazuki T. Sequencing and population analysis of four novel HLA-DPA1 alleles. Human Immunology. 1992 Jan 1;35(3):173-178. https://doi.org/10.1016/0198-8859(92)90102-S
Harada, Haruhito ; Kimura, Akinori ; Dong, Rui Ping ; Xu, Xing Pei ; Bhatia, Kuldeep ; Sasazuki, Takehiko. / Sequencing and population analysis of four novel HLA-DPA1 alleles. In: Human Immunology. 1992 ; Vol. 35, No. 3. pp. 173-178.
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