Sequential actions of glucose oxidase and peroxidase in molecular films assembled by layer-by-layer alternate adsorption

Mitsuhiko Onda, Yuri Lvov, Katsuhiko Ariga, Toyoki Kunitake

Research output: Contribution to journalArticle

253 Citations (Scopus)

Abstract

Molecular films of protein/polyion layers were assembled by means of alternate adsorption through electrostatic interaction. Glucose oxidase (GOD) and peroxidase (POD) were assembled in combination with sodium poly(styrenesulfonate) (PSS) and poly(ethyleneimine) (PEI), respectively. Enzyme activities of those films on specific substrates (glucose and H2O2) were examined by coloring reaction of dye DA67. A multienzyme film containing GOD layer and POD layer was prepared by alternate adsorption of POD/PSS followed by PEI/GOD. Sequential redox reaction of glucose/H2O2/DA67 was demonstrated successfully with this supramolecular system.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalBiotechnology and Bioengineering
Volume51
Issue number2
DOIs
Publication statusPublished - Jul 20 1996
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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