Sequential actions of immune effector cells induced by viral activation of dendritic cells to eliminate murine neuroblastoma

Naonori Kawakubo, Sakura Tanaka, Yoshiaki Kinoshita, Tatsuro Tajiri, Yoshikazu Yonemitsu, Tomoaki Taguchi

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Purpose: In preclinical trails, we reported the antitumor effect of dendritic cells activated with Sendai virus (rSeV/DC) combined with γ-irradiation against neuroblastoma. However, what kind of effector cells for the combined therapy were used to show the antitumor effect was unclear. In this study, we performed radiation and rSeV/DC therapy in vivo and examined the effector cells involved. Methods: Dendritic cells were cultured from bone marrow cells, activated with SeV and administered intratumorally at 106 weekly for 3 weeks. Radiation was administered at 4 Gy/time × 3 times. During the treatment, CD4 + and CD8 + cells and natural killer (NK) cells were removed by antibodies. Results: Complete remission of neuroblastoma was observed in 62.5% of individuals in the combined therapy group. By depleting the effector cells using antibodies, the tumor increased in size from an early stage of treatment in the CD4 + and NK cell-depleted group. In contrast, the tumor increased in size in the late stage of treatment in the CD8 + cell-depleted group. Conclusion: The combination of radiation and rSeV/DC therapy induces different effector cells, depending on the time point during treatment. Level of Evidence: V.

Original languageEnglish
Pages (from-to)1615-1620
Number of pages6
JournalJournal of Pediatric Surgery
Volume53
Issue number8
DOIs
Publication statusPublished - Aug 2018

All Science Journal Classification (ASJC) codes

  • Surgery
  • Pediatrics, Perinatology, and Child Health

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