Sequential analysis of T cells in the liver during murine listerial infection

K. Hiromatsu, G. Matsuzaki, Y. Tauchi, Y. Yoshikai, K. Nomoto

    Research output: Contribution to journalArticle

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    Abstract

    Phenotypes and functions of T cells in the liver were studied after an i.p. inoculation with viable Listeria monocytogenes in mice. T cells in the liver of untreated C3H/HeN mice (C3H; H-2(k), Mls-2a contain Thy-1.2+TCR- αβ+ cells as a majority and Thy-1.2+TCR-γδ+ cells and Thy-1.2-TCR- γδ+ cells as minorities. The liver of untreated C3H mice did not contain T cells expressing Vβ3 and Vβ11, which are potentially autoreactive against self-superantigens of Mls-2a and Dvb1, respectively. On days 3 to 6 after infection, Thy-1.2-CD4(low)TCR-αβ+ T cells or Thy-1.2-TCR-γδ+ T cells increased significantly in number and proportion in the liver whereas T cells with these phenotypes were hardly detected in the spleen, lymph nodes, peripheral blood, and peritoneal cavity during the course of the infection. The Thy-1.2-CD4(low)TCR-αβ T cells contained Vβ3 or Vβ11-bearing cells in high frequencies. The potentially autoreactive Vβ3- or Vβ11-bearing T cells disappeared from the liver on day 7 after infection. Furthermore, the Vβ3+ and Vβ11+ cells but not Vβ8+ cells disappeared after culture for 24 h at 37°C. In vitro stimulation of liver T cells using anti-Vβ11 mAb showed no proliferative response. These results suggest that the potentially autoreactive clones with Thy-1.2-CD4(low) phenotypes, which increased in number after listerial infection, may be anergized after interaction with self-Ag and may be programmed to die. These potentially autoreactive clones induced in the liver of Listeria-infected mice may not be functionally relevant to the host defense against Listeria.

    Original languageEnglish
    Pages (from-to)568-573
    Number of pages6
    JournalJournal of Immunology
    Volume149
    Issue number2
    Publication statusPublished - Jan 1 1992

    Fingerprint

    T-Lymphocytes
    Liver
    Infection
    Listeria
    Inbred C3H Mouse
    Phenotype
    Clone Cells
    Superantigens
    Peritoneal Cavity
    Listeria monocytogenes
    Spleen
    Lymph Nodes

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Hiromatsu, K., Matsuzaki, G., Tauchi, Y., Yoshikai, Y., & Nomoto, K. (1992). Sequential analysis of T cells in the liver during murine listerial infection. Journal of Immunology, 149(2), 568-573.

    Sequential analysis of T cells in the liver during murine listerial infection. / Hiromatsu, K.; Matsuzaki, G.; Tauchi, Y.; Yoshikai, Y.; Nomoto, K.

    In: Journal of Immunology, Vol. 149, No. 2, 01.01.1992, p. 568-573.

    Research output: Contribution to journalArticle

    Hiromatsu, K, Matsuzaki, G, Tauchi, Y, Yoshikai, Y & Nomoto, K 1992, 'Sequential analysis of T cells in the liver during murine listerial infection', Journal of Immunology, vol. 149, no. 2, pp. 568-573.
    Hiromatsu K, Matsuzaki G, Tauchi Y, Yoshikai Y, Nomoto K. Sequential analysis of T cells in the liver during murine listerial infection. Journal of Immunology. 1992 Jan 1;149(2):568-573.
    Hiromatsu, K. ; Matsuzaki, G. ; Tauchi, Y. ; Yoshikai, Y. ; Nomoto, K. / Sequential analysis of T cells in the liver during murine listerial infection. In: Journal of Immunology. 1992 ; Vol. 149, No. 2. pp. 568-573.
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    abstract = "Phenotypes and functions of T cells in the liver were studied after an i.p. inoculation with viable Listeria monocytogenes in mice. T cells in the liver of untreated C3H/HeN mice (C3H; H-2(k), Mls-2a contain Thy-1.2+TCR- αβ+ cells as a majority and Thy-1.2+TCR-γδ+ cells and Thy-1.2-TCR- γδ+ cells as minorities. The liver of untreated C3H mice did not contain T cells expressing Vβ3 and Vβ11, which are potentially autoreactive against self-superantigens of Mls-2a and Dvb1, respectively. On days 3 to 6 after infection, Thy-1.2-CD4(low)TCR-αβ+ T cells or Thy-1.2-TCR-γδ+ T cells increased significantly in number and proportion in the liver whereas T cells with these phenotypes were hardly detected in the spleen, lymph nodes, peripheral blood, and peritoneal cavity during the course of the infection. The Thy-1.2-CD4(low)TCR-αβ T cells contained Vβ3 or Vβ11-bearing cells in high frequencies. The potentially autoreactive Vβ3- or Vβ11-bearing T cells disappeared from the liver on day 7 after infection. Furthermore, the Vβ3+ and Vβ11+ cells but not Vβ8+ cells disappeared after culture for 24 h at 37°C. In vitro stimulation of liver T cells using anti-Vβ11 mAb showed no proliferative response. These results suggest that the potentially autoreactive clones with Thy-1.2-CD4(low) phenotypes, which increased in number after listerial infection, may be anergized after interaction with self-Ag and may be programmed to die. These potentially autoreactive clones induced in the liver of Listeria-infected mice may not be functionally relevant to the host defense against Listeria.",
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