Sequential appearance of γ/δ‐ and α/β‐bearing T cells in the peritoneal cavity during an i.p. infection with Listeria monocytogenes

Shouichi Ohga, Yasunobu Yoshikai, Yasuyuki Takeda, Kenji Hiromatsu, Kikuo Nomoto

Research output: Contribution to journalArticlepeer-review

125 Citations (Scopus)

Abstract

To search for a potential role of T cell antigen receptor (TcR) γ/β‐bearing cells in host‐defense against Listeria monocytogenes, we analyzed the sequential appearance of γ/δ and α/β T cell in the peritoneal exudate cells (PEC) during an i.p. infection with sublethal dose (2 × 103) of viable Listeria organisms in mice. The PEC on day 1 after the infection consisted of 48% macrophages and 50% lymphocytes, most of which were surface IgM+ (B) cells. The number of PEC increased to the maximal level by day 3. The PEC at this stage contained an appreciable number of CD3+ T cells in addition to a large number of macrophages. Of the CD3+ cells, the proportion of CD4CD8 cells, most of which expressed no TcR α/β, increased to the maximal level on day 3 after the infection. In correlation with an increased number of CD3+CD4CD8TcR α/β cells, high level of TcR γ/δ chain gene messages was detected in the nonadherent population of the PEC on this stage. On the other hand, the PEC on day 8 contained an increased number of CD4+CD8 and CD4CD8+ cells which expressed TcR α/β chain on their surface. These results suggest that the γ/δ T cells precede the α/β T cells in appearance during listerial infection. The γ/β T cells may be involved at the first line of the host‐defense against Listeria.

Original languageEnglish
Pages (from-to)533-538
Number of pages6
JournalEuropean Journal of Immunology
Volume20
Issue number3
DOIs
Publication statusPublished - Mar 1990

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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