Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus

A. Iwasaki, Y. Yoshikai, M. Sakumoto, K. Himeno, H. Yuuki, M. Kumamoto, K. Sueishi, K. Nomoto

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    Abstract

    To elucidate the abnormality of T cell differentiation in nude mice grafted with rat fetal thymus that develop multiple-organ-localized autoimmune diseases, we examined sequential appearance of T cell subsets and expression of TCR genes in BALB/c nude mice after grafting with fetal F344 rat thymus. We observed progressive expression of TCRγ/δ-α/β genes in the lymph node (LN) cells from 8 to 12 wk after grafting. An appreciable number of CD4+ T cells but few CD8+ T cells were detected in the LN at 8 wk after grafting. CD8+ T cells increased slowly in number by 12 wk after grafting but remained at a low level in comparison with those in nude mice 12 wk after grafting with BALB/c thymus. In correlation with an increase in the number of T cells expressing TCR α/β genes, alloreactivity as assessed by MLR was increased to a normal level. However, CTL activity against alloantigens remained at a low level in the LN cells at 12 wk. At this stage, organ-specific autoimmune diseases and a high level of anti-DNA autoantibodies were detected. In these mice host-reactive T cells such as Vβ3- or Vβ11-bearing T cells were virtually eliminated in the peripheral mature T cell pool, whereas T cells maturing in the fetal rat thymus significantly proliferated in response to donor-rat stimulator cells. These results suggest that the development of the autoimmune diseases may be ascribed to an impaired maturation of CD8+ T cells but not to failure in clonal elimination of host-reactive T cells in nude mice grafted with rat thymus.

    Original languageEnglish
    Pages (from-to)28-35
    Number of pages8
    JournalJournal of Immunology
    Volume145
    Issue number1
    Publication statusPublished - Jan 1 1990

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

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  • Cite this

    Iwasaki, A., Yoshikai, Y., Sakumoto, M., Himeno, K., Yuuki, H., Kumamoto, M., Sueishi, K., & Nomoto, K. (1990). Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus. Journal of Immunology, 145(1), 28-35.