Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus

A. Iwasaki, Yasunobu Yoshikai, M. Sakumoto, K. Himeno, H. Yuuki, M. Kumamoto, K. Sueishi, K. Nomoto

    Research output: Contribution to journalArticle

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    Abstract

    To elucidate the abnormality of T cell differentiation in nude mice grafted with rat fetal thymus that develop multiple-organ-localized autoimmune diseases, we examined sequential appearance of T cell subsets and expression of TCR genes in BALB/c nude mice after grafting with fetal F344 rat thymus. We observed progressive expression of TCRγ/δ-α/β genes in the lymph node (LN) cells from 8 to 12 wk after grafting. An appreciable number of CD4+ T cells but few CD8+ T cells were detected in the LN at 8 wk after grafting. CD8+ T cells increased slowly in number by 12 wk after grafting but remained at a low level in comparison with those in nude mice 12 wk after grafting with BALB/c thymus. In correlation with an increase in the number of T cells expressing TCR α/β genes, alloreactivity as assessed by MLR was increased to a normal level. However, CTL activity against alloantigens remained at a low level in the LN cells at 12 wk. At this stage, organ-specific autoimmune diseases and a high level of anti-DNA autoantibodies were detected. In these mice host-reactive T cells such as Vβ3- or Vβ11-bearing T cells were virtually eliminated in the peripheral mature T cell pool, whereas T cells maturing in the fetal rat thymus significantly proliferated in response to donor-rat stimulator cells. These results suggest that the development of the autoimmune diseases may be ascribed to an impaired maturation of CD8+ T cells but not to failure in clonal elimination of host-reactive T cells in nude mice grafted with rat thymus.

    Original languageEnglish
    Pages (from-to)28-35
    Number of pages8
    JournalJournal of Immunology
    Volume145
    Issue number1
    Publication statusPublished - Jan 1 1990

    Fingerprint

    T-Lymphocyte Subsets
    Nude Mice
    Thymus Gland
    T-Lymphocytes
    Autoimmune Diseases
    Lymph Nodes
    Gene Expression
    Isoantigens
    Inbred F344 Rats
    Autoantibodies
    Cell Differentiation

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Iwasaki, A., Yoshikai, Y., Sakumoto, M., Himeno, K., Yuuki, H., Kumamoto, M., ... Nomoto, K. (1990). Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus. Journal of Immunology, 145(1), 28-35.

    Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus. / Iwasaki, A.; Yoshikai, Yasunobu; Sakumoto, M.; Himeno, K.; Yuuki, H.; Kumamoto, M.; Sueishi, K.; Nomoto, K.

    In: Journal of Immunology, Vol. 145, No. 1, 01.01.1990, p. 28-35.

    Research output: Contribution to journalArticle

    Iwasaki, A, Yoshikai, Y, Sakumoto, M, Himeno, K, Yuuki, H, Kumamoto, M, Sueishi, K & Nomoto, K 1990, 'Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus', Journal of Immunology, vol. 145, no. 1, pp. 28-35.
    Iwasaki A, Yoshikai Y, Sakumoto M, Himeno K, Yuuki H, Kumamoto M et al. Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus. Journal of Immunology. 1990 Jan 1;145(1):28-35.
    Iwasaki, A. ; Yoshikai, Yasunobu ; Sakumoto, M. ; Himeno, K. ; Yuuki, H. ; Kumamoto, M. ; Sueishi, K. ; Nomoto, K. / Sequential appearance of host-derived T cell subsets during differentiation in nude mice grafted with rat fetal thymus. In: Journal of Immunology. 1990 ; Vol. 145, No. 1. pp. 28-35.
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