TY - JOUR
T1 - Sequential changes in the non-coding control region sequences of JC polyomaviruses from the cerebrospinal fluid of patients with progressive multifocal leukoencephalopathy
AU - Nakamichi, Kazuo
AU - Kishida, Shuji
AU - Tanaka, Kozue
AU - Suganuma, Akihiko
AU - Sano, Yasuteru
AU - Sano, Hironori
AU - Kanda, Takashi
AU - Maeda, Norihisa
AU - Kira, Jun ichi
AU - Itoh, Ai
AU - Kato, Natsuko
AU - Tomimoto, Hidekazu
AU - Kurane, Ichiro
AU - Lim, Chang Kweng
AU - Mizusawa, Hidehiro
AU - Saijo, Masayuki
PY - 2013
Y1 - 2013
N2 - Progressive multifocal leukoencephalopathy (PML) is caused by JC polyomavirus (JCV) infection in the brain. JCV isolates from PML patients have variable mutations in the non-coding control region (NCCR) of the genome. This study was conducted to examine sequential changes in NCCR patterns of JCV isolates obtained from the cerebrospinal fluid (CSF) of PML patients. CSF specimens were collected from PML patients at different time points, the NCCR sequences were determined, and their compositions were assessed by computer-based analysis. In patients showing a marked increase in JCV load, the most frequent NCCR sequences in the follow-up specimens were different from those in the initial samples. In contrast, the dominant NCCRs in the CSF remained unaltered during the follow-up of individuals in whom the viral load decreased after therapeutic intervention. These data demonstrate that the majority of JCV variants emerge with the progression of PML and that these changes are suppressed when the viral load is decreased.
AB - Progressive multifocal leukoencephalopathy (PML) is caused by JC polyomavirus (JCV) infection in the brain. JCV isolates from PML patients have variable mutations in the non-coding control region (NCCR) of the genome. This study was conducted to examine sequential changes in NCCR patterns of JCV isolates obtained from the cerebrospinal fluid (CSF) of PML patients. CSF specimens were collected from PML patients at different time points, the NCCR sequences were determined, and their compositions were assessed by computer-based analysis. In patients showing a marked increase in JCV load, the most frequent NCCR sequences in the follow-up specimens were different from those in the initial samples. In contrast, the dominant NCCRs in the CSF remained unaltered during the follow-up of individuals in whom the viral load decreased after therapeutic intervention. These data demonstrate that the majority of JCV variants emerge with the progression of PML and that these changes are suppressed when the viral load is decreased.
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U2 - 10.1007/s00705-012-1532-3
DO - 10.1007/s00705-012-1532-3
M3 - Article
C2 - 23138154
AN - SCOPUS:84874654606
VL - 158
SP - 639
EP - 650
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 3
ER -