Sequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin

Toshiki Tsurimoto, Thomas Melendy, Bruce Stillman

Research output: Contribution to journalArticlepeer-review

307 Citations (Scopus)

Abstract

Enzymatic synthesis of DNA from the simian virus 40 origin of DNA replication has been reconstituted in vitro with eight purified components. DNA polymerase α-primase complex first initiates DNA synthesis at the replication origin and continues as the lagging strand polymerase. Subsequently, the DNA polymerase δ complex initiates replication on the leading strand template. Some prokaryotic DNA polymerase complexes can replace the eukaryotic polymerase δ complex. A model for polymerase switching during initiation of DNA replication is presented.

Original languageEnglish
Pages (from-to)534-539
Number of pages6
JournalNature
Volume346
Issue number6284
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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