Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter

Mitsuhiko Onda, Yuri Lvov, Katsuhiko Ariga, Toyoki Kunitake

Research output: Contribution to journalArticle

181 Citations (Scopus)

Abstract

Separate molecular layers of glucoamylase (GA) and glucose oxidase (GOD) were assembled on an ultrafilter by layer-by-layer adsorption using poly(ethyleneimine) (PEI) as a polycation. An aqueous starch solution was placed on the ultrafilter which was covered by GA and GOD layers in various sequential arrangements with intervening polyion complex layers of PEI and poly(styrenesulfonate) (PSS). GA hydrolyzed starch to glucose, which was then converted to gluconic acid and H2O2 by GOD. The films with an upper GA layer and a lower GOD layer showed higher overall activities, consistent with the order of the sequential enzymatic reactions. Reaction products and unreacted starch were readily separated by filtration when the substrate solution was passed through these multi-enzyme films.

Original languageEnglish
Pages (from-to)502-506
Number of pages5
JournalJournal of Fermentation and Bioengineering
Volume82
Issue number5
DOIs
Publication statusPublished - Jan 1 1996

Fingerprint

Glucan 1,4-alpha-Glucosidase
Glucose Oxidase
Glucose oxidase
Starch
Polyetherimides
Reaction products
Adsorption
Glucose
Enzymes
Acids
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter. / Onda, Mitsuhiko; Lvov, Yuri; Ariga, Katsuhiko; Kunitake, Toyoki.

In: Journal of Fermentation and Bioengineering, Vol. 82, No. 5, 01.01.1996, p. 502-506.

Research output: Contribution to journalArticle

Onda, Mitsuhiko ; Lvov, Yuri ; Ariga, Katsuhiko ; Kunitake, Toyoki. / Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter. In: Journal of Fermentation and Bioengineering. 1996 ; Vol. 82, No. 5. pp. 502-506.
@article{26d53af4383d45978068db1fb077f8a9,
title = "Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter",
abstract = "Separate molecular layers of glucoamylase (GA) and glucose oxidase (GOD) were assembled on an ultrafilter by layer-by-layer adsorption using poly(ethyleneimine) (PEI) as a polycation. An aqueous starch solution was placed on the ultrafilter which was covered by GA and GOD layers in various sequential arrangements with intervening polyion complex layers of PEI and poly(styrenesulfonate) (PSS). GA hydrolyzed starch to glucose, which was then converted to gluconic acid and H2O2 by GOD. The films with an upper GA layer and a lower GOD layer showed higher overall activities, consistent with the order of the sequential enzymatic reactions. Reaction products and unreacted starch were readily separated by filtration when the substrate solution was passed through these multi-enzyme films.",
author = "Mitsuhiko Onda and Yuri Lvov and Katsuhiko Ariga and Toyoki Kunitake",
year = "1996",
month = "1",
day = "1",
doi = "10.1016/S0922-338X(97)86992-9",
language = "English",
volume = "82",
pages = "502--506",
journal = "Journal of Bioscience and Bioengineering",
issn = "1389-1723",
publisher = "The Society for Biotechnology, Japan",
number = "5",

}

TY - JOUR

T1 - Sequential reaction and product separation on molecular films of glucoamylase and glucose oxidase assembled on an ultrafilter

AU - Onda, Mitsuhiko

AU - Lvov, Yuri

AU - Ariga, Katsuhiko

AU - Kunitake, Toyoki

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Separate molecular layers of glucoamylase (GA) and glucose oxidase (GOD) were assembled on an ultrafilter by layer-by-layer adsorption using poly(ethyleneimine) (PEI) as a polycation. An aqueous starch solution was placed on the ultrafilter which was covered by GA and GOD layers in various sequential arrangements with intervening polyion complex layers of PEI and poly(styrenesulfonate) (PSS). GA hydrolyzed starch to glucose, which was then converted to gluconic acid and H2O2 by GOD. The films with an upper GA layer and a lower GOD layer showed higher overall activities, consistent with the order of the sequential enzymatic reactions. Reaction products and unreacted starch were readily separated by filtration when the substrate solution was passed through these multi-enzyme films.

AB - Separate molecular layers of glucoamylase (GA) and glucose oxidase (GOD) were assembled on an ultrafilter by layer-by-layer adsorption using poly(ethyleneimine) (PEI) as a polycation. An aqueous starch solution was placed on the ultrafilter which was covered by GA and GOD layers in various sequential arrangements with intervening polyion complex layers of PEI and poly(styrenesulfonate) (PSS). GA hydrolyzed starch to glucose, which was then converted to gluconic acid and H2O2 by GOD. The films with an upper GA layer and a lower GOD layer showed higher overall activities, consistent with the order of the sequential enzymatic reactions. Reaction products and unreacted starch were readily separated by filtration when the substrate solution was passed through these multi-enzyme films.

UR - http://www.scopus.com/inward/record.url?scp=0030471615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030471615&partnerID=8YFLogxK

U2 - 10.1016/S0922-338X(97)86992-9

DO - 10.1016/S0922-338X(97)86992-9

M3 - Article

AN - SCOPUS:0030471615

VL - 82

SP - 502

EP - 506

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -