TY - JOUR
T1 - Ser7 of RNAPII-CTD facilitates heterochromatin formation by linking ncRNA to RNAi
AU - Kajitani, Takuya
AU - Kato, Hiroaki
AU - Chikashige, Yuji
AU - Tsutsumi, Chihiro
AU - Hiraoka, Yasushi
AU - Kimura, Hiroshi
AU - Ohkawa, Yasuyuki
AU - Obuse, Chikashi
AU - Hermand, Damien
AU - Murakami, Yota
N1 - Funding Information:
supported by a Grant-in-Aid for Scientific Research (A) from the Japan Society for the Promotion of Science and by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science, and
Funding Information:
Technology of Japan (MEXT). H. Kato was supported by the Japan Science and Technology Agency program Precursory Research for Embryonic Science and Technology and MEXT Grant-in-Aid for Scientific Research 26116513.
PY - 2017/12/26
Y1 - 2017/12/26
N2 - Some long noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (RNAPII) are retained on chromatin, where they regulate RNAi and chromatin structure. The molecular basis of this retention remains unknown. We show that in fission yeast serine 7 (Ser7) of the C-terminal domain (CTD) of RNAPII is required for efficient siRNA generation for RNAi-dependent heterochromatin formation. Surprisingly, Ser7 facilitates chromatin retention of nascent heterochromatic RNAs (hRNAs). Chromatin retention of hRNAs and siRNA generation requires both Ser7 and an RNA-binding activity of the chromodomain of Chp1, a subunit of the RNA-induced transcriptional silencing (RITS) complex. Furthermore, RITS associates with RNAPII in a Ser7-dependent manner. We propose that Ser7 promotes cotranscriptional chromatin retention of hRNA by recruiting the RNA-chromatin connector protein Chp1, which facilitates RNAi-dependent heterochromatin formation. Our findings reveal a function of the CTD code: linking ncRNA transcription to RNAi for heterochromatin formation.
AB - Some long noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (RNAPII) are retained on chromatin, where they regulate RNAi and chromatin structure. The molecular basis of this retention remains unknown. We show that in fission yeast serine 7 (Ser7) of the C-terminal domain (CTD) of RNAPII is required for efficient siRNA generation for RNAi-dependent heterochromatin formation. Surprisingly, Ser7 facilitates chromatin retention of nascent heterochromatic RNAs (hRNAs). Chromatin retention of hRNAs and siRNA generation requires both Ser7 and an RNA-binding activity of the chromodomain of Chp1, a subunit of the RNA-induced transcriptional silencing (RITS) complex. Furthermore, RITS associates with RNAPII in a Ser7-dependent manner. We propose that Ser7 promotes cotranscriptional chromatin retention of hRNA by recruiting the RNA-chromatin connector protein Chp1, which facilitates RNAi-dependent heterochromatin formation. Our findings reveal a function of the CTD code: linking ncRNA transcription to RNAi for heterochromatin formation.
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U2 - 10.1073/pnas.1714579115
DO - 10.1073/pnas.1714579115
M3 - Article
C2 - 29237752
AN - SCOPUS:85039723343
SN - 0027-8424
VL - 114
SP - E11208-E11217
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 52
ER -